PMID- 21397022
OWN - NLM
STAT- MEDLINE
DCOM- 20110801
LR  - 20110531
IS  - 1879-1220 (Electronic)
IS  - 0960-0760 (Linking)
VI  - 125
IP  - 3-5
DP  - 2011 Jul
TI  - Complex organization of the 5'-untranslated region of the mouse estrogen receptor 
      alpha gene: identification of numerous mRNA transcripts with distinct 5'-ends.
PG  - 211-8
LID - 10.1016/j.jsbmb.2011.03.004 [doi]
AB  - The 5'-untranslated region (5'-UTR) of the estrogen receptor alpha (ERalpha) gene plays 
      an important role in determining its tissue-specific expression. We examined the 
      5'-UTRs of the mouse ERalpha mRNA variants in depth using the Basic Local Alignment 
      Search Tool (BLAST), rapid amplification of 5'-cDNA ends (5'-RACE) and RT-PCR. We 
      demonstrated the presence of multiple variants containing unique 5'-UTRs. We 
      mapped the cDNA sequences onto the mouse genome, and found that both alternative 
      splicing from four different leader exons (A, C, F1, and H) to exon 1, and 
      combinations of 12 internal exons (X1, X2, X3, X4, F2/X5, X6, X7, X8, X9, X10, 
      X11, and B) generate multiple ERalpha transcripts. Mouse exon B, that has homologies 
      with human exon B and rat exon 0T, was used as an internal exon, not as a leader 
      exon. RT-PCR analysis revealed distinct expression patterns of the variants, 
      suggesting that the alternative promoter usage and alternative splicing are 
      regulated in a tissue-specific manner. Our results indicate that the genomic 
      organization of the mouse ERalpha gene is complicated as previously shown in the rat 
      ERalpha gene. In addition, both alternative promoter usage and alternative splicing 
      contribute to the remarkable mRNA diversity of the mouse ERalpha gene.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Ishii, Hirotaka
AU  - Ishii H
AD  - Department of Physiology, Nippon Medical School, 1 Sendagi, Bunkyo, Tokyo 
      113-8602, Japan. hirotaka@nms.ac.jp
FAU - Sakuma, Yasuo
AU  - Sakuma Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110321
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Animals
MH  - Blotting, Southern
MH  - Estrogen Receptor alpha/*genetics
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
EDAT- 2011/03/15 06:00
MHDA- 2011/08/02 06:00
CRDT- 2011/03/15 06:00
PHST- 2010/08/16 00:00 [received]
PHST- 2011/02/18 00:00 [revised]
PHST- 2011/03/04 00:00 [accepted]
PHST- 2011/03/15 06:00 [entrez]
PHST- 2011/03/15 06:00 [pubmed]
PHST- 2011/08/02 06:00 [medline]
AID - S0960-0760(11)00047-1 [pii]
AID - 10.1016/j.jsbmb.2011.03.004 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2011 Jul;125(3-5):211-8. doi: 
      10.1016/j.jsbmb.2011.03.004. Epub 2011 Mar 21.