PMID- 21397592
OWN - NLM
STAT- MEDLINE
DCOM- 20110808
LR  - 20211020
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1388
DP  - 2011 May 4
TI  - NMDA receptor subunit expression in the supraoptic nucleus of adult rats: 
      dominance of NR2B and NR2D.
PG  - 89-99
LID - 10.1016/j.brainres.2011.03.015 [doi]
AB  - The supraoptic nucleus (SON) of the hypothalamus contains magnocellular 
      neurosecretory neurons (MNC) which synthesize and release the peptide hormones 
      vasopressin and oxytocin. Glutamate is a prominent excitatory neurotransmitter in 
      the SON and regulates MNC excitability. NMDA receptors (NMDAR), a type of 
      ionotropic glutamate receptor, mediate synaptic plasticity of MNCs and are 
      necessary for characteristic burst firing patterns which serve to maximize 
      hormone release. NMDARs are di- or tri-heteromeric complexes of NR1 and NR2 
      subunits. Receptor properties depend on NR2 subunit composition and variable 
      splicing of NR1. We investigated the expression profile of NR1 and NR2 subunits 
      in the SON at the mRNA and protein levels plus protein expression of NR1 splice 
      variants in control and salt-loaded adult rats. There was robust mRNA expression 
      of all subunits, with NR2D levels being the highest. At the protein level, NR1, 
      NR2B, and NR2D were robustly expressed, while NR2A was weakly expressed. NR2C 
      protein was not detected with either of the two antibodies tested. All four NR1 
      splice variant cassettes (N1, C1, C2, C2') were detected in the SON, although NR1 
      N1 expression was too low for accurate analysis. Three days of salt-loading did 
      not alter mRNA, protein, or splice variant expression of NMDAR subunits in the 
      SON. Robust NR2D protein expression has not been previously shown in MNCs and is 
      uncommon in the adult brain. Although the functional significance of this unusual 
      expression profile is unknown, it may contribute to important physiological 
      characteristics of SON neurons, such as burst firing and resistance to 
      excitotoxicity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Doherty, Faye C
AU  - Doherty FC
AD  - University of Colorado, School of Medicine, Department of Physiology and 
      Biophysics, RC-1 North Tower, P18-7127, Mail Stop 8307, Aurora, CO 80045, USA. 
      Faye.Doherty@world.oberlin.edu
FAU - Sladek, Celia D
AU  - Sladek CD
LA  - eng
GR  - R01 NS027975/NS/NINDS NIH HHS/United States
GR  - R01 NS027975-18A2/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110331
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (NR2D NMDA receptor)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Dehydration/metabolism
MH  - Gene Expression
MH  - *Gene Expression Profiling
MH  - Male
MH  - Protein Isoforms/biosynthesis
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Supraoptic Nucleus/*metabolism
PMC - PMC3086706
MID - NIHMS281361
EDAT- 2011/03/15 06:00
MHDA- 2011/08/09 06:00
PMCR- 2012/05/04
CRDT- 2011/03/15 06:00
PHST- 2011/02/01 00:00 [received]
PHST- 2011/03/03 00:00 [revised]
PHST- 2011/03/04 00:00 [accepted]
PHST- 2011/03/15 06:00 [entrez]
PHST- 2011/03/15 06:00 [pubmed]
PHST- 2011/08/09 06:00 [medline]
PHST- 2012/05/04 00:00 [pmc-release]
AID - S0006-8993(11)00512-9 [pii]
AID - 10.1016/j.brainres.2011.03.015 [doi]
PST - ppublish
SO  - Brain Res. 2011 May 4;1388:89-99. doi: 10.1016/j.brainres.2011.03.015. Epub 2011 
      Mar 31.