PMID- 21397653
OWN - NLM
STAT- MEDLINE
DCOM- 20110906
LR  - 20190816
IS  - 1096-0295 (Electronic)
IS  - 0273-2300 (Linking)
VI  - 60
IP  - 1
DP  - 2011 Jun
TI  - Protective effects of Chlorella-derived peptide on UVB-induced production of 
      MMP-1 and degradation of procollagen genes in human skin fibroblasts.
PG  - 112-9
LID - 10.1016/j.yrtph.2011.03.001 [doi]
AB  - UV exposure is known to induce premature aging, which is mediated by matrix 
      metalloproteinase-1 (MMP-1) activity. MMP-1 mRNA expression is up-regulated by 
      elevated cysteine-rich 61 (CYR61) and monocyte chemoattractant protein-1 (MCP-1) 
      via action of transcription factor AP-1. Collagen is degraded by MMP-1 activity 
      but synthesized by transforming growth factor-beta (TGF-beta) signal. Chlorella has 
      been shown to inhibit UVB-induced MMP-1 level, however its regulatory molecular 
      mechanisms have not been studied. In this study, Chlorella derived peptide (CDP) 
      was added to skin fibroblasts after UVB irradiation and the expression of MMP-1, 
      CYR61, procollagen, c-fos, c-jun, and TGF-beta receptor (TbRII) mRNA and MCP-1 
      production were investigated. CDP (10 or 5mg/ml) diminished UVB-induced MMP-1 and 
      CYR61 mRNA expression and MCP-1 production, whereas, UVB-suppressed procollagen 
      and TbRII mRNA was restored by CDP treatment. UVB-induced c-fos and c-jun 
      expressions were also inhibited by the CDP treatment. Taken together, CDP 
      inhibits UVB-induced MMP-1 expression in skin fibroblasts by suppressing 
      expression of AP-1 and CYR61 and MCP-1 production.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Chen, Chiu-Lan
AU  - Chen CL
AD  - Department of Pharmacy, Chia-Nan University of Pharmacy & Science, Tainan, 
      Taiwan.
FAU - Liou, Shu-Fen
AU  - Liou SF
FAU - Chen, Su-Jong
AU  - Chen SJ
FAU - Shih, Mei-Fen
AU  - Shih MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110321
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCN1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cysteine-Rich Protein 61)
RN  - 0 (Peptides)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Proteins)
RN  - 0 (Procollagen)
RN  - 0 (Transcription Factor AP-1)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Cell Proliferation/drug effects/radiation effects
MH  - Cell Survival/drug effects/radiation effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chlorella/*chemistry
MH  - Cysteine-Rich Protein 61/genetics/metabolism
MH  - Fibroblasts/*drug effects/metabolism/radiation effects
MH  - Gene Expression/*drug effects/radiation effects
MH  - Humans
MH  - Matrix Metalloproteinase 1/genetics/*metabolism
MH  - Peptides/isolation & purification/*pharmacology
MH  - Plant Extracts/pharmacology
MH  - Plant Proteins/isolation & purification/pharmacology
MH  - Procollagen/genetics/*metabolism/radiation effects
MH  - Skin/*drug effects/metabolism/radiation effects
MH  - Transcription Factor AP-1/genetics/metabolism
MH  - Ultraviolet Rays
EDAT- 2011/03/15 06:00
MHDA- 2011/09/07 06:00
CRDT- 2011/03/15 06:00
PHST- 2010/10/28 00:00 [received]
PHST- 2011/02/08 00:00 [revised]
PHST- 2011/03/02 00:00 [accepted]
PHST- 2011/03/15 06:00 [entrez]
PHST- 2011/03/15 06:00 [pubmed]
PHST- 2011/09/07 06:00 [medline]
AID - S0273-2300(11)00054-7 [pii]
AID - 10.1016/j.yrtph.2011.03.001 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2011 Jun;60(1):112-9. doi: 10.1016/j.yrtph.2011.03.001. 
      Epub 2011 Mar 21.