PMID- 21398279
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20181201
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 52
IP  - 8
DP  - 2011 Jul 29
TI  - Characterization of conditioned media collected from aged versus young human eye 
      cups.
PG  - 5963-72
LID - 10.1167/iovs.10-6440 [doi]
AB  - PURPOSE: To characterize secretion of in situ retinal pigment epithelium (RPE) 
      from healthy, aged adult, age-related macular degeneration (AMD) adult, and fetal 
      donor eyes and to assess the impact on retinal survival in vitro. METHODS: 
      Conditioned medium (CM) was collected from adult and fetal donor eyes and 
      analyzed for trophic factor composition by multiplex ELISA. Trophic factor 
      receptor occupancy was calculated to evaluate differences in trophic factor 
      concentrations. RPE trophic factor mRNA expression was quantified by real-time 
      PCR. Retina-preserving activity of the collected CM was evaluated using 
      degenerating porcine retina in vitro. RESULTS: Compared with CM from adult 
      donors, AMD donor CM contained a significantly higher concentration of 
      brain-derived neurotrophic factor (BDNF), whereas fetal donor CM contained 
      significantly higher concentrations of hepatocyte growth factor (HGF) and pigment 
      epithelium-derived factor (PEDF). No consistent correlation was found between 
      trophic factor mRNA expression and protein secretion. Non-RPE components of the 
      RPE-Bruch's membrane-choroid-sclera complex were major contributors of vascular 
      endothelial growth factor-A (VEGF-A). CM of fetal donors was significantly better 
      than CM of adult or AMD donors at improving the survival of degenerating porcine 
      retina. CONCLUSIONS: RPE cells of adult and fetal eyes have significantly 
      different trophic factor production capabilities, which correlated with changes 
      in preservation of porcine retina. Combined with trophic factor receptor 
      occupancy calculations, these data may implicate HGF and PEDF as key factors 
      promoting the preservation of retinal structure and function.
FAU - Kolomeyer, Anton M
AU  - Kolomeyer AM
AD  - Institute of Ophthalmology and Visual Science, University of Medicine and 
      Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey 07101, 
      USA.
FAU - Sugino, Ilene K
AU  - Sugino IK
FAU - Zarbin, Marco A
AU  - Zarbin MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110729
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Growth Factor)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Aging/*pathology
MH  - Animals
MH  - Apoptosis/physiology
MH  - Cell Survival/physiology
MH  - Choroid/cytology/metabolism
MH  - Culture Media, Conditioned/*metabolism
MH  - Eye Banks
MH  - Female
MH  - Fetus/cytology/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Macular Degeneration/metabolism/*pathology
MH  - Male
MH  - Models, Biological
MH  - Organ Culture Techniques
MH  - RNA, Messenger/metabolism
MH  - Receptors, Growth Factor/metabolism
MH  - Retinal Pigment Epithelium/*cytology/metabolism/*pathology
MH  - Sclera/cytology/metabolism
MH  - Swine
MH  - Tissue Donors
MH  - Tissue Preservation
EDAT- 2011/03/15 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/03/15 06:00
PHST- 2011/03/15 06:00 [entrez]
PHST- 2011/03/15 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - iovs.10-6440 [pii]
AID - 10.1167/iovs.10-6440 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2011 Jul 29;52(8):5963-72. doi: 10.1167/iovs.10-6440.