PMID- 2140076
OWN - NLM
STAT- MEDLINE
DCOM- 19900618
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 36
IP  - 5
DP  - 1990 May
TI  - Evaluation of the RheumaStrip ANA profile test: a rapid test strip procedure for 
      simultaneously determining antibodies to autoantigens U1-ribonucleoprotein 
      (U1-RNP), Sm, SS-A/Ro, SS-B/La, and to native DNA.
PG  - 792-7
AB  - The "LipoGen RheumaStrip ANA Profile" test method (LipoGen, Inc.) is a new assay 
      format for autoantibody detection in which recombinant autoantigens are used. 
      This enzyme immunoassay, in test-strip format, detects antibodies to autoantigens 
      U1-ribonucleoprotein (U1-RNP), Sm, SS-A/Ro, SS-B/La, and to native DNA (nDNA). We 
      evaluated 200 antinuclear antibody (ANA)-positive and 100 ANA-negative sera for 
      the presence of antibodies to U1-RNP, Sm, SS-A/Ro, SS-B/La, and nDNA by the new 
      test-strip procedure. These data correlated well with those obtained with either 
      Ouchterlony double immunodiffusion for U1-RNP, Sm, SS-A/Ro, and SS-B/La or with 
      Crithidia luciliae indirect immunofluorescence for anti-nDNA. Assay sensitivity 
      and assay specificity of the ANA Profile method as compared with those of 
      established procedures were respectively as follows: 89.8% and 98.8% for U1-RNP, 
      86.4% and 95.3% for Sm, 97.9% and 89.3% for SS-A/Ro, 98.3% and 86.3% for SS-B/La, 
      and 97.5% and 93.1% for nDNA. Agreement between the ANA Profile test and these 
      other test methodologies ranged from 88.7% for the SS-B/La test to 97.3% for the 
      U1-RNP test. This new test procedure substantially decreases the time and effort 
      required to perform these assays. Total hands-on time and overall assay time were 
      decreased by 72% and 97%, respectively.
FAU - Paxton, H
AU  - Paxton H
AD  - Maryland Medical Laboratory, Inc., Baltimore 21227.
FAU - Bendele, T
AU  - Bendele T
FAU - O'Connor, L
AU  - O'Connor L
FAU - Haynes, D C
AU  - Haynes DC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Antibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Reagent Strips)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
SB  - IM
MH  - Antibodies/*analysis
MH  - Autoantigens/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunodiffusion
MH  - Precipitin Tests
MH  - *Reagent Strips
MH  - Ribonucleoproteins/*immunology
MH  - Ribonucleoproteins, Small Nuclear
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1990 May;36(5):792-7.