PMID- 21408193
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 3
DP  - 2011 Mar 7
TI  - A large expansion of the HSFY gene family in cattle shows dispersion across Yq 
      and testis-specific expression.
PG  - e17790
LID - 10.1371/journal.pone.0017790 [doi]
LID - e17790
AB  - Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock 
      transcriptional factor (HSF) family that is found in multiple copies on the Y 
      chromosome and conserved in a number of species. Its function still remains 
      unknown but in humans it is thought to play a role in spermatogenesis. Through 
      real time polymerase chain reaction (PCR) analyses we determined that the HSFY 
      family is largely expanded in cattle ( approximately 70 copies) compared with human (2 
      functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does 
      not vary among individual bulls as a copy number variant (CNV). Using 
      fluorescence in situ hybridization (FISH) we found that the copies are dispersed 
      along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears 
      restricted to the testis and its mRNA correlates positively with mRNA markers of 
      spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which 
      suggests that HSFY is expressed (at least in part) in early germ cells.
FAU - Hamilton, Christine K
AU  - Hamilton CK
AD  - Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada.
FAU - Revay, Tamas
AU  - Revay T
FAU - Domander, Robin
AU  - Domander R
FAU - Favetta, Laura A
AU  - Favetta LA
FAU - King, W Allan
AU  - King WA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110307
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calibration
MH  - Cattle/*genetics
MH  - DNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Electrophoresis, Agar Gel
MH  - Female
MH  - Gene Dosage/genetics
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Heat Shock Transcription Factors
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Multigene Family/*genetics
MH  - Organ Specificity/genetics
MH  - Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Testis/*metabolism
MH  - Transcription Factors/chemistry/*genetics/metabolism
MH  - Y Chromosome/*genetics
PMC - PMC3049798
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/03/17 06:00
MHDA- 2011/07/06 06:00
PMCR- 2011/03/07
CRDT- 2011/03/17 06:00
PHST- 2010/10/30 00:00 [received]
PHST- 2011/02/10 00:00 [accepted]
PHST- 2011/03/17 06:00 [entrez]
PHST- 2011/03/17 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
PHST- 2011/03/07 00:00 [pmc-release]
AID - PONE-D-10-05333 [pii]
AID - 10.1371/journal.pone.0017790 [doi]
PST - epublish
SO  - PLoS One. 2011 Mar 7;6(3):e17790. doi: 10.1371/journal.pone.0017790.