PMID- 21411785
OWN - NLM
STAT- MEDLINE
DCOM- 20110512
LR  - 20110317
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 135
IP  - 4
DP  - 2011 Apr
TI  - The diagnostic utility of p16 FISH and GLUT-1 immunohistochemical analysis in 
      mesothelial proliferations.
PG  - 619-27
LID - 10.1309/AJCPP5R2ZJZKCLWN [doi]
AB  - Two promising ancillary tests used in the diagnosis of mesothelioma include 
      GLUT-1 immunohistochemical analysis and fluorescence in situ hybridization (FISH) 
      testing for the p16 deletion. This study compared the diagnostic usefulness of 
      p16 FISH and GLUT-1 immunohistochemical analysis in the diagnosis of mesothelial 
      proliferations in 158 cases with a diagnosis of benign (45.4%), atypical (10.4%), 
      or malignant/mesothelioma (44.2%). Of the 70 benign cases, none had a deletion of 
      p16 and 5 cases (7%) were positive for GLUT-1. Of the 68 mesotheliomas, 40 (59%) 
      had a deletion of p16 (sensitivity, 59%; specificity, 100%) and 27 (40%) were 
      positive for GLUT-1 (sensitivity, 40%; specificity, 93%). GLUT-1 showed lower 
      sensitivity in pleural (56% vs 70%) and peritoneal (29% vs 51%) mesotheliomas (P 
      = .004). Our results demonstrate that p16 FISH is a more sensitive and specific 
      test than GLUT-1 immunohistochemical analysis and can be a more reliable 
      ancillary tool to support the diagnosis of mesothelioma.
FAU - Monaco, Sara E
AU  - Monaco SE
AD  - Dept of Pathology, University of Pittsburgh Medical Center, PA 15232, USA.
FAU - Shuai, Yongli
AU  - Shuai Y
FAU - Bansal, Mona
AU  - Bansal M
FAU - Krasinskas, Alyssa M
AU  - Krasinskas AM
FAU - Dacic, Sanja
AU  - Dacic S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Glucose Transporter Type 1)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Proliferation
MH  - Epithelium/metabolism/pathology
MH  - Female
MH  - *Gene Deletion
MH  - *Genes, p16
MH  - Glucose Transporter Type 1/*metabolism
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesothelioma/*diagnosis/genetics/metabolism
MH  - Middle Aged
MH  - Peritoneal Neoplasms/*diagnosis/genetics/metabolism
MH  - Pleural Neoplasms/*diagnosis/genetics/metabolism
MH  - Predictive Value of Tests
MH  - Young Adult
EDAT- 2011/03/18 06:00
MHDA- 2011/05/13 06:00
CRDT- 2011/03/18 06:00
PHST- 2011/03/18 06:00 [entrez]
PHST- 2011/03/18 06:00 [pubmed]
PHST- 2011/05/13 06:00 [medline]
AID - 135/4/619 [pii]
AID - 10.1309/AJCPP5R2ZJZKCLWN [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2011 Apr;135(4):619-27. doi: 10.1309/AJCPP5R2ZJZKCLWN.