PMID- 2141302
OWN - NLM
STAT- MEDLINE
DCOM- 19900726
LR  - 20190918
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 11
IP  - 2-3
DP  - 1990 Feb-Mar
TI  - Imaging [Ca2+]i dynamics during signal transduction.
PG  - 145-55
AB  - The elevation of free intracellular Ca2+ activity ([Ca2+]i) is widely recognised 
      as a central event in many signal transduction processes in cellular physiology. 
      Recent advances in optical techniques for measuring [Ca2+]i as well as 
      developments in quantitative low light level fluorescence microscopy have led to 
      the application of these methods to the study of subcellular [Ca2+]i in many 
      biological systems. In the following paper we describe some techniques in our 
      laboratory to provide quantitative high spatio-temporal resolution measurements 
      of [Ca2+]i in individual living cells during the signal transduction of cell 
      surface receptor ligand interactions. In particular, we are studying the changes 
      in [Ca2+]i induced by the micro-aggregation of immunoglobulin E (IgE) receptor 
      complexes on the surface of rat basophilic leukemia (RBL) cells (a tumor mast 
      cell line) by multivalent antigen. We seek to understand the mechanisms which are 
      involved in the detection of these cell surface events which lead to changes in 
      [Ca2+]i as well as the interactions between the various subcellular components 
      which impart the delicate control of [Ca2+]i during cellular stimulation. The 
      limitations and properties of the technology used for these studies will be 
      discussed, and some illustrative examples of the type of [Ca2+]i changes found in 
      this biological system will be given.
FAU - Ryan, T A
AU  - Ryan TA
AD  - Department of Physics, Cornell University, Ithaca, New York.
FAU - Millard, P J
AU  - Millard PJ
FAU - Webb, W W
AU  - Webb WW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Antigens)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Benzofurans)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Antigens
MH  - Antigens, Differentiation, B-Lymphocyte/metabolism
MH  - Benzofurans
MH  - Calcium/*metabolism
MH  - Fura-2
MH  - Image Processing, Computer-Assisted
MH  - Immunoglobulin E/metabolism
MH  - Leukemia, Basophilic, Acute
MH  - Rats
MH  - Receptor Aggregation
MH  - Receptors, Fc/metabolism
MH  - Receptors, IgE
MH  - *Signal Transduction
MH  - Tumor Cells, Cultured
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - 0143-4160(90)90067-5 [pii]
AID - 10.1016/0143-4160(90)90067-5 [doi]
PST - ppublish
SO  - Cell Calcium. 1990 Feb-Mar;11(2-3):145-55. doi: 10.1016/0143-4160(90)90067-5.