PMID- 21414236
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20211020
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 12
DP  - 2011 Mar 18
TI  - CTLA4 is expressed on mature dendritic cells derived from human monocytes and 
      influences their maturation and antigen presentation.
PG  - 21
LID - 10.1186/1471-2172-12-21 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) initiate immune responses through their direct 
      interaction with effector cells. However, the mechanism by which DC activity is 
      regulated is not well defined. Previous studies have shown that CTLA4 on T cells 
      regulates DCs function by "cross-talk". We investigated whether there is an 
      intrinsic regulatory mechanism in DCs, with CTLA4 as a candidate regulator. 
      RESULTS: We confirmed via RT-PCR and flow cytometry the natural expression of 
      CTLA4 on mature DCs derived from human monocytes. Approximately 8% CD1a-positive 
      cells express CTLA4 both on surface and intracellular, whereas 10% CD1a-negative 
      cells express CTLA4 intracellularly, but little expression was observed on the 
      cell surface. The cross-linking of CTLA4 inhibits DCs maturation and antigen 
      presentation in vitro, but does not inhibit endocytosis. CONCLUSIONS: CTLA4 is 
      expressed by DCs and plays an inhibitory role. CTLA4-expressing DCs may represent 
      a group of regulatory DCs. Because of its wide distribution on different cell 
      types, CTLA4 may play a general role in regulating immune responses.
FAU - Wang, Xiong B
AU  - Wang XB
AD  - Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China. xiongbiao6@yahoo.com
FAU - Fan, Zhong Z
AU  - Fan ZZ
FAU - Anton, Doina
AU  - Anton D
FAU - Vollenhoven, Annika V
AU  - Vollenhoven AV
FAU - Ni, Zhen H
AU  - Ni ZH
FAU - Chen, Xiao F
AU  - Chen XF
FAU - Lefvert, Ann K
AU  - Lefvert AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110318
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD/genetics/*immunology/metabolism
MH  - Antigens, CD1/immunology/metabolism
MH  - CTLA-4 Antigen
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Endocytosis/immunology
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/cytology/*immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3070687
EDAT- 2011/03/19 06:00
MHDA- 2011/06/24 06:00
PMCR- 2011/03/18
CRDT- 2011/03/19 06:00
PHST- 2010/04/15 00:00 [received]
PHST- 2011/03/18 00:00 [accepted]
PHST- 2011/03/19 06:00 [entrez]
PHST- 2011/03/19 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
PHST- 2011/03/18 00:00 [pmc-release]
AID - 1471-2172-12-21 [pii]
AID - 10.1186/1471-2172-12-21 [doi]
PST - epublish
SO  - BMC Immunol. 2011 Mar 18;12:21. doi: 10.1186/1471-2172-12-21.