PMID- 21415532
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20220317
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 34
IP  - 2
DP  - 2011
TI  - Curcumin attenuates lipopolysaccharide-induced renal inflammation.
PG  - 226-32
AB  - Renal inflammation is the main pathological change in many acute and chronic 
      kidney diseases. Curcumin, a yellow pigment present in the rhizome of turmeric 
      (Curcuma longa L. Zingiberaceae), was found to be a potential anti-inflammatory 
      agent. The present study aimed to investigate the effects of curcumin on the 
      inflammation of mice kidney and cultured renal tubular epithelial cells (HK-2 
      cells) induced by lipopolysaccharide (LPS) and to explore the mechanism. Curcumin 
      was injected intraperitoneally before LPS administration. Renal inflammation was 
      assessed by evaluating monocyte chemoattractant protein-1 (MCP-1) expression and 
      macrophage infiltration in renal tissue using immunohistochemical methods, and 
      also by measuring renal MCP-1 mRNA level using Real-Time polymerase chain 
      reaction (PCR). HK-2 cells were cultured to investigate the in vitro effect of 
      curcumin against LPS-induced renal inflammation. The expression of MCP-1 and 
      interleukin-8 (IL-8) mRNA was measured by Real-Time PCR. The expression of MCP-1 
      and IL-8 protein in supernatant was detected by enzyme-linked immunosorbent assay 
      (ELISA). The activity of nuclear factor (NF)-kappaB was detected by electrophoretic 
      mobility shift assay (EMSA). The results demonstrated that curcumin could inhibit 
      LPS-induced renal MCP-1 mRNA expression. Curcumin also significantly inhibited 
      the expression of MCP-1 and IL-2 mRNA in HK-2 cells, and partially inhibited the 
      secretion of MCP-1 and IL-8. Furthermore, curcumin was found to inhibit the 
      DNA-binding activity of NF-kappaB. The present study demonstrated that curcumin has a 
      protective effect on LPS-induced experimental renal inflammation, and this effect 
      might be attributed to its inhibitory effects on MCP-1 mRNA expression and 
      DNA-binding activity of NF-kappaB. Hence, curcumin might be potentially useful in 
      some kidney diseases by preventing renal inflammation.
FAU - Zhong, Fang
AU  - Zhong F
AD  - Department of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University, School 
      of Medicine, Shanghai 200025, PR China.
FAU - Chen, Hui
AU  - Chen H
FAU - Han, Lin
AU  - Han L
FAU - Jin, Yuanmeng
AU  - Jin Y
FAU - Wang, Weiming
AU  - Wang W
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/*therapeutic use
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Curcuma/*chemistry
MH  - Curcumin/pharmacology/*therapeutic use
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Interleukin-2/genetics/metabolism
MH  - Interleukin-8/genetics/metabolism
MH  - Lipopolysaccharides
MH  - Mice
MH  - Mice, Inbred Strains
MH  - NF-kappa B/metabolism
MH  - Nephritis/metabolism/*prevention & control
MH  - *Phytotherapy
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Rhizome
EDAT- 2011/03/19 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/03/19 06:00
PHST- 2011/03/19 06:00 [entrez]
PHST- 2011/03/19 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
AID - JST.JSTAGE/bpb/34.226 [pii]
AID - 10.1248/bpb.34.226 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2011;34(2):226-32. doi: 10.1248/bpb.34.226.