PMID- 21415591
OWN - NLM
STAT- MEDLINE
DCOM- 20111026
LR  - 20211109
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 43
IP  - 4
DP  - 2011 Apr 30
TI  - Cathepsin B is activated as an executive protease in fetal rat alveolar type II 
      cells exposed to hyperoxia.
PG  - 223-9
AB  - Alveolar type II cells are main target of hyperoxia-induced lung injury. The 
      authors investigated whether lysosomal protease, cathepsin B (CB), is activated 
      in fetal alveolar type II cells in the transitional period from the canalicular 
      to saccular stages during 65%-hyperoxia and whether CB is related to fetal 
      alveolar type II cell (FATIIC) death secondary to hyperoxia. FATIICs were 
      isolated from embryonic day 19 rats and exposed to 65%-oxygen for 24 h and 36 h. 
      The cells exposed to room air were used as controls. Cell cytotoxicity was 
      assessed by lactate dehydrogenase-release and flow cytometry, and apoptosis was 
      analyzed by TUNEL assay and flow cytometry. CB activity was assessed by 
      colorimetric assay, qRT-PCR and western blots. 65%-hyperoxia induced FATIIC death 
      via necrosis and apoptosis. Interestingly, caspase-3 activities were not enhanced 
      in FATIICs during 65%-hyperoxia, whereas CB activities were greatly increased 
      during 65%-hyperoxia in a time-dependent manner, and similar findings were 
      observed with qRT-PCR and western blots. In addition, the preincubation of CB 
      inhibitor prior to 65%-hyperoxia reduced FATIIC death significantly. Our studies 
      suggest that CB activation secondary to hyperoxia might have a relevant role in 
      executing the cell death program in FATIICs during the acute stage of 
      65%-hyperoxia.
FAU - Lee, Hyeon-Soo
AU  - Lee HS
AD  - Department of Pediatrics, Kangwon National University Hospital, Kangwon Naitonal 
      University School of Medicine, Chuncheon, Korea. premee@kangwon.ac.kr
FAU - Kim, Chun-Ki
AU  - Kim CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.1 (Cathepsin B)
RN  - EC 3.4.22.1 (Ctsb protein, rat)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Alveolar Epithelial Cells/cytology/*metabolism
MH  - Animals
MH  - Caspase 3
MH  - Cathepsin B/*metabolism
MH  - Cell Death
MH  - Cell Hypoxia
MH  - Enzyme Activation
MH  - Female
MH  - In Situ Nick-End Labeling
MH  - L-Lactate Dehydrogenase/analysis
MH  - Lung/metabolism
MH  - Necrosis/metabolism
MH  - Oxygen
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Pulmonary Alveoli/cytology/embryology/*enzymology
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC3085741
EDAT- 2011/03/19 06:00
MHDA- 2011/10/27 06:00
PMCR- 2011/04/30
CRDT- 2011/03/19 06:00
PHST- 2011/03/19 06:00 [entrez]
PHST- 2011/03/19 06:00 [pubmed]
PHST- 2011/10/27 06:00 [medline]
PHST- 2011/04/30 00:00 [pmc-release]
AID - emm.2010.43.027 [pii]
AID - 10.3858/emm.2011.43.4.027 [doi]
PST - ppublish
SO  - Exp Mol Med. 2011 Apr 30;43(4):223-9. doi: 10.3858/emm.2011.43.4.027.