PMID- 21416058
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 3
IP  - 2
DP  - 2011 Feb
TI  - Validation of a novel, physiologic model of experimental acute pancreatitis in 
      the mouse.
PG  - 159-65
AB  - BACKGROUND: Many experimental models of acute pancreatitis suffer from lack of 
      clinical relevance. We sought to validate a recently reported murine model of 
      acute pancreatitis that more closely represents the physiology of human biliary 
      pancreatitis. METHODS: Mice (C57BL/6J n=6 and CF-1 n=8) underwent infusion of 
      50mul of 5% sodium taurocholate (NaT) or 50mul of normal saline (NaCl) directly 
      into the pancreatic duct. Twenty-four hours later, pancreatitis severity was 
      graded histologically by three independent observers, and pancreatic tissue 
      concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 
      (MCP-1) were determined by ELISA. RESULTS: Twenty four hours after retrograde 
      injection, the total pancreatitis score was significantly greater in mice infused 
      with NaT than in those infused with NaCl (6.3 +/- 1.2 vs. 1.2 +/- 0.4, p<0.05). In 
      addition, the inflammatory mediators IL-6 and MCP-1 were increased in the NaT 
      group relative to the NaCl group. DISCUSSION: Retrograde pancreatic duct infusion 
      of sodium taurocholate induces acute pancreatitis in the mouse. This model is 
      likely representative of human biliary pancreatitis pathophysiology, and 
      therefore provides a powerful tool with which to elucidate basic mechanisms 
      underlying the pathogenesis of acute pancreatitis.
FAU - Ziegler, Kathryn M
AU  - Ziegler KM
AD  - Department of Surgery, Indiana University Indianapolis, IN, USA.
FAU - Wade, Terence E
AU  - Wade TE
FAU - Wang, Sue
AU  - Wang S
FAU - Swartz-Basile, Deborah A
AU  - Swartz-Basile DA
FAU - Pitt, Henry A
AU  - Pitt HA
FAU - Zyromski, Nicholas J
AU  - Zyromski NJ
LA  - eng
PT  - Journal Article
DEP - 20101223
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC3056562
OTO - NOTNLM
OT  - Acute pancreatitis
OT  - animal model
OT  - murine
OT  - retrograde injection
OT  - sodium taurocholate
EDAT- 2011/03/19 06:00
MHDA- 2011/03/19 06:01
PMCR- 2010/12/23
CRDT- 2011/03/19 06:00
PHST- 2010/11/23 00:00 [received]
PHST- 2010/12/20 00:00 [accepted]
PHST- 2011/03/19 06:00 [entrez]
PHST- 2011/03/19 06:00 [pubmed]
PHST- 2011/03/19 06:01 [medline]
PHST- 2010/12/23 00:00 [pmc-release]
PST - ppublish
SO  - Am J Transl Res. 2011 Feb;3(2):159-65. Epub 2010 Dec 23.