PMID- 21416360
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110318
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 7
DP  - 1991
TI  - Use of vectors to confer resistance to antibiotics g418 and hygromycin in stably 
      transfected cell lines.
PG  - 245-56
LID - 10.1385/0-89603-178-0:245 [doi]
AB  - The development of dominant selection markers to identify eukaryotic cells that 
      have undergone a gene transformation event has greatly facilitated molecular 
      genetic studies in higher eukaryotic cells. Selection schemes based on resistance 
      to antibiotic cytotoxicity (1,2) will be described in this chapter. Other 
      schemes-for example, based on resistance to inhibition of DNA synthesis by 
      methotrexate (1) or mycophenolic acid (1)-are described in other chapters of this 
      book. Prior to the development of dominant selection markers, the use of 
      recessive markers, such as thymidine kinase (TK) or hypoxanthine-guanine 
      phosphoribosyl transferase (HGPT) was limited to a handful of mutant cell lines 
      that were TK (-) or HGPT(-) (5,6). If one wished to transfect a wild-type cell 
      line, one had first to select a recessive mutant derivative cell line and 
      characterize it before proceeding with the experiments of interest. Such 
      restrictions posed a significant barrier to molecular genetic analyses in higher 
      eukaryotic cells.
FAU - Santerre, R F
AU  - Santerre RF
AD  - Department of Molecular Biology, Lilly Reserch Laboratories, Indianapolis, IN.
FAU - Walls, J D
AU  - Walls JD
FAU - Grinnell, B W
AU  - Grinnell BW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 2011/03/19 06:00
PHST- 2011/03/19 06:00 [entrez]
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
AID - 10.1385/0-89603-178-0:245 [doi]
PST - ppublish
SO  - Methods Mol Biol. 1991;7:245-56. doi: 10.1385/0-89603-178-0:245.