PMID- 21417824
OWN - NLM
STAT- MEDLINE
DCOM- 20111024
LR  - 20190116
IS  - 1029-2403 (Electronic)
IS  - 1026-8022 (Linking)
VI  - 52
IP  - 6
DP  - 2011 Jun
TI  - High rate of neoplastic cells with genetic abnormalities in proliferation centers 
      of chronic lymphocytic leukemia.
PG  - 1080-4
LID - 10.3109/10428194.2011.555889 [doi]
AB  - In lymph nodes, chronic lymphocytic leukemia (CLL) cells (prolymphocytes and 
      paraimmunoblasts) form proliferation centers (PCs), which are also known as 
      pseudofollicles. To reveal whether PCs play a role in the accumulation of genetic 
      alterations in CLL, we compared deletion at 11q22.3, 13q14.3, and 17p13.1 loci 
      and trisomy 12 by the fluorescence in situ hybridization (FISH) technique in PCs 
      versus surrounding small lymphocytes (SLs) in 12 formalin-fixed paraffin-embedded 
      (FFPE) lymph nodes. The FFPE sections were stained with methylene blue and PCs 
      were marked by laser beam. Subsequent FISH analysis was performed, relocalizing 
      the previously defined regions. Loss of 11q was detected in five cases, loss of 
      13q in two cases, loss of 17p in two cases, and trisomy 12 in one case. In seven 
      cases PCs contained a significantly higher ratio of cells with genetic 
      alterations compared with the surrounding SLs. Our results show that CLL cells 
      with genetic alterations tend to accumulate in PCs. The clonal expansion of the 
      cell population carrying genetic alterations within PCs may contribute to CLL 
      progression.
FAU - Balogh, Zsofia
AU  - Balogh Z
AD  - 1st Department of Pathology and Experimental Cancer Research, Semmelweis 
      University, Budapest, Hungary.
FAU - Reiniger, Lilla
AU  - Reiniger L
FAU - Rajnai, Hajnalka
AU  - Rajnai H
FAU - Csomor, Judit
AU  - Csomor J
FAU - Szepesi, Agota
AU  - Szepesi A
FAU - Balogh, Aniko
AU  - Balogh A
FAU - Deak, Linda
AU  - Deak L
FAU - Gagyi, Eva
AU  - Gagyi E
FAU - Bodor, Csaba
AU  - Bodor C
FAU - Matolcsy, Andras
AU  - Matolcsy A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110321
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
SB  - IM
CIN - Leuk Lymphoma. 2011 Jun;52(6):946-7. PMID: 21534869
MH  - *Cell Proliferation
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 12/genetics
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Lymph Nodes/*metabolism/pathology
MH  - Lymphocytes/metabolism/pathology
MH  - Trisomy
EDAT- 2011/03/23 06:00
MHDA- 2011/10/25 06:00
CRDT- 2011/03/23 06:00
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/10/25 06:00 [medline]
AID - 10.3109/10428194.2011.555889 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2011 Jun;52(6):1080-4. doi: 10.3109/10428194.2011.555889. Epub 
      2011 Mar 21.