PMID- 21418993
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20171116
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 91
IP  - 2
DP  - 2011 Jan 11
TI  - [Effects of advanced glycation end products on the secretion of proinflammatory 
      chemokines in vascular smooth muscle cells].
PG  - 107-10
AB  - OBJECTIVE: To investigate the effects of advanced glycation end products (AGEs) 
      on the secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 
      (IL-8) in vascular smooth muscle cells (VSMCs) and explore its possible 
      intracellular signaling mechanism. METHODS: Primary rat VSMCs were isolated and 
      identified. VSMCs were treated with glycation serum albumin (GSA), an important 
      component of AGEs, in series of concentrations and time. The role of MAPK and 
      NF-kappaB inhibitors was confirmed. The levels of MCP-1 and IL-8 were determined by 
      enzyme-linked immunosorbent assay (ELISA). RESULTS: VSMCs were treated with GSA 
      at the doses of 10 microg/ml, 100 microg/ml and 500 microg/ml respectively. In comparison 
      with the control group, the levels of MCP-1 (13.01 ng/ml +/- 0.12 ng/ml vs 7.02 
      ng/ml +/- 0.26 ng/ml, P < 0.05) and IL-8 (12.6 ng/ml +/- 0.86 ng/ml vs 3.07 ng/ml +/- 
      0.35 ng/ml, P < 0.05) increased in the GSA-treated group, especially at the 
      concentration of 100 microg/ml. After adjustment for cells proliferation, the levels 
      of MCP-1 and IL-8 were still higher in the GSA-treated group. After a 
      pretreatment of PDTC (10 micromol/L), SB203580 (5 micromol/L) and MG132 (10 micromol/L), the 
      levels of MCP-1 and IL-8 decreased. However, it had no change when pretreated 
      with PD98059 (20 micromol/L). CONCLUSION: GSA promotes the secretion of MCP-1 and 
      IL-8 in VSMCs. Such an effect is not dependent on cellular proliferation. It may 
      be realized through an activation of NF-kappaB by p38MAPK-sensitive intracellular 
      signaling pathway.
FAU - He, Rong
AU  - He R
AD  - Department of Cardiology, Peking University Third Hospital, Key Laboratory of 
      Molecular Cardiovascular Sciences Ministry of Education, Beijing 100191, China.
FAU - Mao, Jie-Ming
AU  - Mao JM
FAU - Wang, Guang
AU  - Wang G
FAU - Gao, Wei
AU  - Gao W
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*metabolism
MH  - Glycation End Products, Advanced/*pharmacology
MH  - Interleukin-8/*metabolism
MH  - Male
MH  - Muscle, Smooth, Vascular/cytology/drug effects/*metabolism
MH  - Myocytes, Smooth Muscle/drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2011/03/23 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/03/23 06:00
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2011 Jan 11;91(2):107-10.