PMID- 21420147
OWN - NLM
STAT- MEDLINE
DCOM- 20110613
LR  - 20161125
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 409
IP  - 11
DP  - 2011 May 1
TI  - In vitro steroidogenic effects of mixtures of persistent organic pollutants 
      (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes.
PG  - 2040-8
LID - 10.1016/j.scitotenv.2011.01.055 [doi]
AB  - This study investigated the effects of two mixtures of persistent organic 
      pollutants (POPs) on steroidogenesis in the H295R cell line. The two mixtures 
      were obtained from the livers of burbot (Lota lota) caught in two Norwegian lakes 
      (Mjosa and Losna) with different contaminant profiles. Steroid hormone levels in 
      the cell culture medium and mRNA levels of 16 genes involved in steroidogenesis 
      were investigated. The crude Lake Mjosa extract had to be diluted ten times more 
      than the Lake Losna extract in order to prevent cytotoxicity. The ten times 
      diluted Lake Mjosa mixture had higher levels of DDT and derivates ( summation operatorDDTs, 1.7 
      times) and brominated flame retardants ( summation operatorBDEs and HBCD, 15-25 times) than the 
      Lake Losna mixture, which, on the other hand, had higher concentrations of  summation operatorPCBs 
      (1.5 times higher) and also of HCB,  summation operatorHCH isomers and  summation operatorchlordane isomers (5-20 
      times higher). In the cell culture media, only cortisol levels were increased at 
      the highest exposure concentration to the Lake Mjosa mixture, while both cortisol 
      and estradiol levels were increased following exposure to the two highest Lake 
      Losna mixture exposure concentrations. Testosterone levels decreased only at the 
      highest exposure concentration of the Lake Losna mixture. Multivariate models 
      suggested that  summation operatorPCBs, and to a lesser extent  summation operatorDDTs, were responsible for the 
      cortisol responses, while estradiol and testosterone alterations were best 
      explained by HCB and  summation operatorPCBs, respectively. Exposure to the mixtures generally 
      increased mRNA levels, with smaller effects exerted by the Lake Mjosa mixture 
      than the Lake Losna mixture. It was concluded that both mixtures affected 
      steroidogenesis in the H295R cells. Small differences in mixture composition, 
      rather than the high content of brominated flame retardants in the Lake Mjosa 
      mixture, were suggested to be the most probable reason for the apparent 
      differences in potencies of the two mixtures.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Zimmer, Karin E
AU  - Zimmer KE
AD  - Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary 
      Science, Oslo, Norway.
FAU - Montano, Mauricio
AU  - Montano M
FAU - Olsaker, Ingrid
AU  - Olsaker I
FAU - Dahl, Ellen
AU  - Dahl E
FAU - Berg, Vidar
AU  - Berg V
FAU - Karlsson, Camilla
AU  - Karlsson C
FAU - Murk, Albertinka J
AU  - Murk AJ
FAU - Skaare, Janneche U
AU  - Skaare JU
FAU - Ropstad, Erik
AU  - Ropstad E
FAU - Verhaegen, Steven
AU  - Verhaegen S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110321
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Flame Retardants)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Water Pollutants, Chemical)
RN  - 5I9835JO3M (hexabromocyclododecane)
RN  - CIW5S16655 (DDT)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - DDT/metabolism
MH  - Endocrine Disruptors/*metabolism/toxicity
MH  - Environmental Monitoring
MH  - Flame Retardants/metabolism
MH  - Fresh Water/*chemistry
MH  - Gadiformes/blood/*metabolism/physiology
MH  - Gene Expression/drug effects
MH  - Gonadal Steroid Hormones/*blood
MH  - Halogenated Diphenyl Ethers/metabolism
MH  - Hydrocarbons, Brominated/metabolism
MH  - Norway
MH  - Water Pollutants, Chemical/*metabolism/toxicity
EDAT- 2011/03/23 06:00
MHDA- 2011/06/15 06:00
CRDT- 2011/03/23 06:00
PHST- 2010/09/24 00:00 [received]
PHST- 2011/01/24 00:00 [revised]
PHST- 2011/01/27 00:00 [accepted]
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/06/15 06:00 [medline]
AID - S0048-9697(11)00114-8 [pii]
AID - 10.1016/j.scitotenv.2011.01.055 [doi]
PST - ppublish
SO  - Sci Total Environ. 2011 May 1;409(11):2040-8. doi: 
      10.1016/j.scitotenv.2011.01.055. Epub 2011 Mar 21.