PMID- 21420725
OWN - NLM
STAT- MEDLINE
DCOM- 20120110
LR  - 20110718
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 128
IP  - 2
DP  - 2011 Aug
TI  - Gene expression profiling reveals multiple differences in platelets from patients 
      with stable angina or non-ST elevation acute coronary syndrome.
PG  - 161-8
LID - 10.1016/j.thromres.2011.02.012 [doi]
AB  - INTRODUCTION: Platelets play a key role in coronary artery disease. They have the 
      capacity of protein synthesis through translation of megakaryocyte-derived mRNAs, 
      which may influence pathophysiological functions. The present study aimed to 
      prove the concept that platelets from patients with non-ST elevation acute 
      coronary syndrome (NSTE-ACS) have differential mRNA expression profiles, in the 
      hypothesis that this may influence their thrombogenicity. MATERIALS AND METHODS: 
      Gene expression profiles were determined in RNA pools from resting platelets of 
      patients with stable angina (SA, n = 14) or NSTE-ACS (n = 15) using a glass 
      microarray platform. Validation was done by real-time PCR and immunoblot analyses 
      in independent sets of individual samples (26 SA and 17 NSTE-ACS patients, in 
      total). Parallel comparison with healthy subjects was performed to relate the 
      relative abundance of validated genes in CAD patients to a control expression 
      level. RESULTS: Microarray analysis identified 45 transcripts with a significant 
      >/= +/- 2.0-fold difference in expression between NSTE-ACS and SA platelet pools. 
      Thus, gene expression profiles at least partially discriminate unstable from 
      stable CAD. Validation confirmed a significant over-expression of 3 genes in 
      NSTE-ACS at both mRNA and protein level. In particular, the glycoprotein Ib 
      beta-polypeptide (GP1BB) was increased in NSTE-ACS also in comparison with healthy 
      subjects. CONCLUSION: This study provides evidence that NSTE-ACS platelets are 
      potentially preconditioned to a higher degree of reactivity on the 
      transcriptional level. Our data suggest that a different composition of the mRNA 
      pool might mediate an increased platelet prothrombotic potential in NSTE-ACS 
      patients.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Colombo, Gualtiero
AU  - Colombo G
AD  - Centro Cardiologico Monzino IRCCS, Via Parea 4, Milan, Italy.
FAU - Gertow, Karl
AU  - Gertow K
FAU - Marenzi, Giancarlo
AU  - Marenzi G
FAU - Brambilla, Marta
AU  - Brambilla M
FAU - De Metrio, Monica
AU  - De Metrio M
FAU - Tremoli, Elena
AU  - Tremoli E
FAU - Camera, Marina
AU  - Camera M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110321
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Acute Coronary Syndrome/*blood/*genetics
MH  - Aged
MH  - Angina, Stable/*blood/*genetics
MH  - Blood Platelets/metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Platelet Count
MH  - RNA/blood
EDAT- 2011/03/23 06:00
MHDA- 2012/01/11 06:00
CRDT- 2011/03/23 06:00
PHST- 2010/05/11 00:00 [received]
PHST- 2011/02/16 00:00 [revised]
PHST- 2011/02/21 00:00 [accepted]
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2012/01/11 06:00 [medline]
AID - S0049-3848(11)00093-4 [pii]
AID - 10.1016/j.thromres.2011.02.012 [doi]
PST - ppublish
SO  - Thromb Res. 2011 Aug;128(2):161-8. doi: 10.1016/j.thromres.2011.02.012. Epub 2011 
      Mar 21.