PMID- 21421472
OWN - NLM
STAT- MEDLINE
DCOM- 20130305
LR  - 20131121
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 31
IP  - 3
DP  - 2011 Mar
TI  - [Association between cell apoptosis and the quality of early mouse embryos].
PG  - 409-13
AB  - OBJECTIVE: To investigate the relationship between cell apoptosis and the quality 
      of early mouse embryos, understand the significance of apoptosis-regulatory genes 
      in early embryonic development, and explore a new approach to improving the 
      embryo quality. METHODS: The levels of cell apoptosis and proliferation in early 
      mouse embryos in different developmental status (morphologically normal embryos, 
      arrested embryos and fragmented embryos) were analyzed with terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), caspase in 
      situ fluorescence and Bcl-2 immunofluorescence, and immunofluorescent detection 
      of proliferating cell nuclear antigen (PCNA). RESULTS: The cells in arrested 
      embryos and embryonic fragments showed positive results in TUNEL assay with 
      enhanced caspase activity and lowered expressions of Bcl-2 and PCNA. CONCLUSION: 
      Cell apoptosis in early mouse embryos may be closely related to embryonic arrest 
      and fragmentation.
FAU - YU, Min
AU  - YU M
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang 
      Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: 
      yumin1028@163.com.
FAU - QIU, Zhuo-lin
AU  - QIU ZL
FAU - LI, Hong
AU  - LI H
FAU - Zeng, Wei-sen
AU  - Zeng WS
FAU - CHEN, Lei-Ning
AU  - CHEN LN
FAU - LI, Qiu-hua
AU  - LI QH
FAU - QUAN, Song
AU  - QUAN S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 114100-40-2 (Bcl2 protein, mouse)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Caspases/metabolism
MH  - Embryo, Mammalian/*cytology
MH  - Female
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Pregnancy
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2
EDAT- 2011/03/23 06:00
MHDA- 2013/03/06 06:00
CRDT- 2011/03/23 06:00
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2013/03/06 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2011 Mar;31(3):409-13.