PMID- 21423756
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 3
DP  - 2011 Mar 14
TI  - Adipose-derived stem cells stimulate regeneration of peripheral nerves: BDNF 
      secreted by these cells promotes nerve healing and axon growth de novo.
PG  - e17899
LID - 10.1371/journal.pone.0017899 [doi]
LID - e17899
AB  - Transplantation of adipose-derived mesenchymal stem cells (ASCs) induces tissue 
      regeneration by accelerating the growth of blood vessels and nerve. However, 
      mechanisms by which they accelerate the growth of nerve fibers are only partially 
      understood. We used transplantation of ASCs with subcutaneous matrigel implants 
      (well-known in vivo model of angiogenesis) and model of mice limb reinnervation 
      to check the influence of ASC on nerve growth. Here we show that ASCs stimulate 
      the regeneration of nerves in innervated mice's limbs and induce axon growth in 
      subcutaneous matrigel implants. To investigate the mechanism of this action we 
      analyzed different properties of these cells and showed that they express 
      numerous genes of neurotrophins and extracellular matrix proteins required for 
      the nerve growth and myelination. Induction of neural differentiation of ASCs 
      enhances production of brain-derived neurotrophic factor (BDNF) as well as 
      ability of these cells to induce nerve fiber growth. BDNF neutralizing antibodies 
      abrogated the stimulatory effects of ASCs on the growth of nerve sprouts. These 
      data suggest that ASCs induce nerve repair and growth via BDNF production. This 
      stimulatory effect can be further enhanced by culturing the cells in neural 
      differentiation medium prior to transplantation.
FAU - Lopatina, Tatiana
AU  - Lopatina T
AD  - Department of Biochemistry and Molecular Medicine, Faculty of Fundamental 
      Medicine, Lomonosov Moscow State University, Moscow, Russia. t.lopatina@gmail.com
FAU - Kalinina, Natalia
AU  - Kalinina N
FAU - Karagyaur, Maxim
AU  - Karagyaur M
FAU - Stambolsky, Dmitry
AU  - Stambolsky D
FAU - Rubina, Kseniya
AU  - Rubina K
FAU - Revischin, Alexander
AU  - Revischin A
FAU - Pavlova, Galina
AU  - Pavlova G
FAU - Parfyonova, Yelena
AU  - Parfyonova Y
FAU - Tkachuk, Vsevolod
AU  - Tkachuk V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110314
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drug Combinations)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Laminin)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 9007-34-5 (Collagen)
SB  - IM
EIN - PLoS One. 2019 Jul 12;14(7):e0219946. PMID: 31299059
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Axons/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Collagen/metabolism
MH  - Drug Combinations
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - GAP-43 Protein/metabolism
MH  - Humans
MH  - Implants, Experimental
MH  - Laminin/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Fibers/metabolism
MH  - Nerve Growth Factors/metabolism
MH  - Nerve Regeneration/*physiology
MH  - Peroneal Nerve/pathology/physiopathology
MH  - Proteoglycans/metabolism
MH  - Recovery of Function/physiology
MH  - *Stem Cell Transplantation
MH  - Stem Cells/cytology/*metabolism
MH  - *Wound Healing
PMC - PMC3056777
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/03/23 06:00
MHDA- 2011/07/06 06:00
PMCR- 2011/03/14
CRDT- 2011/03/23 06:00
PHST- 2010/12/09 00:00 [received]
PHST- 2011/02/14 00:00 [accepted]
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
PHST- 2011/03/14 00:00 [pmc-release]
AID - PONE-D-10-06499 [pii]
AID - 10.1371/journal.pone.0017899 [doi]
PST - epublish
SO  - PLoS One. 2011 Mar 14;6(3):e17899. doi: 10.1371/journal.pone.0017899.