PMID- 21424240
OWN - NLM
STAT- MEDLINE
DCOM- 20111021
LR  - 20211020
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 47
IP  - 5-6
DP  - 2011 Jun
TI  - Production of functional dendritic cells from menstrual blood--a new dendritic 
      cell source for immune therapy.
PG  - 368-75
LID - 10.1007/s11626-011-9399-2 [doi]
AB  - Dendritic cells (DCs) are the most professional antigen-presenting cells of the 
      mammalian immune system. They are able to phagocytize, process antigen materials, 
      and then present them to the surface of other cells including T lymphocytes in 
      the immune system. These capabilities make DC therapy become a novel and 
      promising immune-therapeutic approach for cancer treatment as well as for cancer 
      vaccination. Many trials of DC therapy to treat cancers have been performed and 
      have shown their application value. They involve harvesting monocytes or 
      hematopoietic stem cells from a patient and processing them in the laboratory to 
      produce DCs and then reintroduced into a patient in order to activate the immune 
      system. DCs were successfully produced from peripheral, umbilical cord 
      blood-derived monocytes or hematopoietic stem cells. In this research, we 
      produced DCs from human menstrual blood-derived monocytes. Briefly, monocytes 
      were isolated by FACS based on FSC vs. SSC plot from lysed menstrual blood. 
      Obtained monocytes were induced into DCs by a two-step protocol. In the first 
      step, monocytes were incubated in RPMI medium supplemented with 2% FBS, GM-CSF, 
      and IL-4, followed by incubation in RPMI medium supplemented with alpha-TNF in the 
      second step. Our data showed that induced monocytes had typical morphology of 
      DCs, expressed HLA-DR, HLA-ABC, CD80 and CD86 markers, exhibited uptake of 
      dextran-FITC, stimulated allogenic T cell proliferation, and released IL-12. 
      These results demonstrated that menstrual blood can not only be a source of 
      stromal stem cell but also DCs, which are a potential candidate for immune 
      therapy.
FAU - Phuc, Pham Van
AU  - Phuc PV
AD  - Laboratory of Stem cell Research and Application, University of Science, Vietnam 
      National University, Ho Chi Minh, Vietnam. pvphuc@hcmuns.edu.vn
FAU - Lam, Dang Hoang
AU  - Lam DH
FAU - Ngoc, Vu Bich
AU  - Ngoc VB
FAU - Thu, Duong Thi
AU  - Thu DT
FAU - Nguyet, Nguyen Thi Minh
AU  - Nguyet NT
FAU - Ngoc, Phan Kim
AU  - Ngoc PK
LA  - eng
PT  - Journal Article
DEP - 20110318
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Antigen-Presenting Cells/immunology/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-12/immunology/metabolism
MH  - Menstrual Cycle/blood/*immunology
MH  - Monocytes/cytology/metabolism
MH  - T-Lymphocytes/immunology/metabolism
EDAT- 2011/03/23 06:00
MHDA- 2011/10/22 06:00
CRDT- 2011/03/23 06:00
PHST- 2010/12/21 00:00 [received]
PHST- 2011/02/22 00:00 [accepted]
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/10/22 06:00 [medline]
AID - 10.1007/s11626-011-9399-2 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2011 Jun;47(5-6):368-75. doi: 
      10.1007/s11626-011-9399-2. Epub 2011 Mar 18.