PMID- 21425182
OWN - NLM
STAT- MEDLINE
DCOM- 20110809
LR  - 20110405
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 29
IP  - 3
DP  - 2011 Apr
TI  - Genome-wide analysis of gene expression in dendritic cells from rat regenerating 
      liver after partial hepatectomy.
PG  - 255-64
LID - 10.1002/cbf.1748 [doi]
AB  - Dendritic cells (DCs) play a pivotal role in orchestrating immune response 
      occurring in liver regeneration (LR). However, there are few details about 
      relationship between DCs and LR at the molecular level. In this study, we firstly 
      obtained high-purity DCs by the combination of Percoll density gradient 
      centrifugation and immunomagentic bead sorting, then measured genome-wide gene 
      expression in DCs from rat regenerating liver after partial hepatectomy (PH) 
      using rat genome 230 2.0 microarray composed of 25,020 genes, and verified the 
      reliability of microarray data with RT-PCR method. Among 25,020 genes present on 
      the array, 1621 known genes and 1307 unknown genes, totally 2928 genes, were 
      identified to be LR-related. H-clustering analysis showed that 2928 genes were 
      grouped into three expression patterns: up-regulation, down-regulation and 
      complex changes in expression. And 1621 known genes were functionally grouped 
      into at least 23 biological categories. When expression patterns were combined 
      with gene functions, as a whole, the genes involved in immune/defence response, 
      inflammatory response and secretion of active substance in DCs were highly 
      enriched in down-regulated pattern. DCs exhibited the reduced immune/defence and 
      inflammatory response, and the suppressed secretion capacity of active substances 
      after PH. A further analysis of genes expressed in the phase-dependent manner 
      during LR suggested the rapid induction of genes encoding some transcription 
      factors and cytokines in DCs at immediate-early phase, the unobvious enhancement 
      of metabolic process, immunity and inflammation at proliferation phase, while the 
      impairment of detoxification, immunity and inflammation at terminal phase.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Xu, Cunshuan
AU  - Xu C
AD  - College of Life Science, Henan Normal University, Xinxiang, China. 
      cellkeylab@126.com
FAU - Chen, Xiaoguang
AU  - Chen X
FAU - Chang, Cuifang
AU  - Chang C
FAU - Wang, Gaiping
AU  - Wang G
FAU - Wang, Wenbo
AU  - Wang W
FAU - Zhang, Lianxing
AU  - Zhang L
FAU - Zhu, Qiushi
AU  - Zhu Q
FAU - Wang, Lei
AU  - Wang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110321
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Antigens, CD)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Cell Count
MH  - Cell Proliferation
MH  - Centrifugation, Density Gradient
MH  - Dendritic Cells/chemistry/*metabolism
MH  - Down-Regulation
MH  - *Gene Expression
MH  - *Gene Expression Profiling
MH  - Genome
MH  - Genome-Wide Association Study
MH  - Hepatectomy
MH  - Immunity/genetics
MH  - Immunohistochemistry
MH  - Immunomagnetic Separation
MH  - Inflammation/*genetics/surgery
MH  - Liver/physiology/surgery
MH  - *Liver Regeneration/genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2011/03/23 06:00
MHDA- 2011/08/10 06:00
CRDT- 2011/03/23 06:00
PHST- 2010/09/29 00:00 [received]
PHST- 2011/01/03 00:00 [revised]
PHST- 2011/02/02 00:00 [accepted]
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/08/10 06:00 [medline]
AID - 10.1002/cbf.1748 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2011 Apr;29(3):255-64. doi: 10.1002/cbf.1748. Epub 2011 Mar 
      21.