PMID- 2142605
OWN - NLM
STAT- MEDLINE
DCOM- 19900830
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 29
IP  - 19
DP  - 1990 May 15
TI  - A nanosecond fluorescence depolarization study on the segmental flexibility of 
      receptor-bound immunoglobulin E.
PG  - 4607-12
AB  - Time-resolved fluorescence anisotropy measurements have been used to examine the 
      segmental flexibility of anti-dansyl immunoglobulin E (IgE) bound to its 
      high-affinity receptor on membrane vesicles from rat basophilic leukemia cells. 
      Although IgE in this complex exhibits only a restricted angular range of 
      segmental motion, much of this restricted motion occurs on a relatively rapid 
      time scale. A fast component of motion with a rotational correlation time of 
      15-35 ns may correspond to the twisting of Fab segments about their major axis. 
      Intermolecular cross-linking by a short bivalent ligand, N,N'-didansylcadaverine, 
      results in complete loss of this segmental motion. Solubilization of monomeric 
      IgE-receptor complexes using a zwitterionic detergent results in a time-dependent 
      anisotropy decay that exhibits both a fast component and a slower component that 
      is intermediate between the decay for soluble and membrane-bound forms of IgE at 
      long times after excitation. These results are discussed in terms of a model in 
      which binding of IgE to its membrane-bound receptor restricts not only its global 
      rotation but also its slower modes of segmental flexibility as well, while 
      allowing its Fab segments to undergo rapid reorientation within a limited angular 
      range.
FAU - Holowka, D
AU  - Holowka D
AD  - Department of Chemistry, Cornell University, Ithaca, New York 14853.
FAU - Wensel, T
AU  - Wensel T
FAU - Baird, B
AU  - Baird B
LA  - eng
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - AI22449/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation, B-Lymphocyte/*metabolism
MH  - Binding Sites, Antibody
MH  - Fluorescence Polarization
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia, Basophilic, Acute/immunology/metabolism
MH  - Motion
MH  - Rats
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
MH  - Solubility
EDAT- 1990/05/15 00:00
MHDA- 1990/05/15 00:01
CRDT- 1990/05/15 00:00
PHST- 1990/05/15 00:00 [pubmed]
PHST- 1990/05/15 00:01 [medline]
PHST- 1990/05/15 00:00 [entrez]
AID - 10.1021/bi00471a015 [doi]
PST - ppublish
SO  - Biochemistry. 1990 May 15;29(19):4607-12. doi: 10.1021/bi00471a015.