PMID- 21426806
OWN - NLM
STAT- MEDLINE
DCOM- 20111017
LR  - 20221207
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 40
IP  - 2
DP  - 2011 Feb
TI  - [Detection of human telomerase RNA component gene by fluorescent in situ 
      hybridization for screening of cervical lesions].
PG  - 104-7
AB  - OBJECTIVE: To investigate the value of fluorescence in situ hybridization (FISH) 
      detection of human telomerase RNA component (hTERC) gene amplification in 
      screening of cervical lesions. METHODS: A total of 146 post-thinPrep cytology 
      test (TCT) samples were analyzed using FISH by two-color interphase probe 
      targeting hTERC gene at chromosome 3q26 and the data were compared with the 
      cytological and histological results. RESULTS: FISH analysis was successful in 
      120 cases (20 cases of normal and 100 abnormal cases by TCT). Gene amplification 
      of hTERC by FISH had a positive correlation with the cytological (r = 0.465, P < 
      0.01) and histological grade results (r = 0.610, P < 0.01). Extra copies of hTERC 
      were seen in 28.6% (6/21) of CINI, 61.1% (11/18) of CINII, 75.0% (18/24) of 
      CINIII and 91.7%(22/24) of squamous cell carcinoma, respectively. None (0/13) of 
      the inflammation cases showed hTERC amplification. The sensitivity and 
      specificity for detecting high grade lesions by FISH were 77.3% (51/66) and 82.4% 
      (28/34); and the positive and negative predictive values were 89.5% and 65.1%, 
      respectively. The rate of hTERC gene gain in high grade lesions was significantly 
      higher than that in the low grade lesions (chi(2) = 32.550, P < 0.01). Combined 
      with the high copy numbers, the sensitivity for detecting high grade lesions was 
      increased to 81.2%. CONCLUSIONS: Detection of hTERC gene amplification by FISH 
      improves the screening efficiency of high-risk cervical epithelial lesions. The 
      presence of high copy numbers of hTERC correlates with the presence of high grade 
      cervical dysplasia.
FAU - Gao, Li
AU  - Gao L
AD  - Department of Pathology, Changhai Hospital, the Second Military Medical 
      University, Shanghai 200433, China.
FAU - Zhu, Yan
AU  - Zhu Y
FAU - Liu, Su-ping
AU  - Liu SP
FAU - Gao, Yan
AU  - Gao Y
FAU - Zhu, Ming-hua
AU  - Zhu MH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/diagnosis/*genetics/pathology
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Neoplasms/diagnosis/*genetics/pathology
MH  - Uterine Cervicitis/diagnosis/genetics/pathology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/diagnosis/*genetics/pathology
EDAT- 2011/03/24 06:00
MHDA- 2011/10/18 06:00
CRDT- 2011/03/24 06:00
PHST- 2011/03/24 06:00 [entrez]
PHST- 2011/03/24 06:00 [pubmed]
PHST- 2011/10/18 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2011 Feb;40(2):104-7.