PMID- 21429313
OWN - NLM
STAT- MEDLINE
DCOM- 20111103
LR  - 20210309
IS  - 0578-1310 (Print)
IS  - 0578-1310 (Linking)
VI  - 49
IP  - 1
DP  - 2011 Jan
TI  - [Effects of edaravone on the expression of GRP78, Caspase-12, and neuron 
      apoptosis in juvenile rat hippocampus after status convulsive].
PG  - 53-9
AB  - OBJECTIVE: To observe the expression of GRP78 (glucose regulated protein, GRP78), 
      Caspase-12 and the change of neuron apoptosis in the juvenile rat hippocampus 
      after status convulsive (SC), and to explore the effect of edaravone on them. 
      METHODS: One hundred and ninety-five juvenile male Sprague-Dawley (SD) rats were 
      randomly divided into normal saline control group (NS group), status convulsive 
      group (SC group) and edaravone treatment group (ED group). Each group was further 
      divided into five subgroups in different executed time points after SC. The rats 
      in status convulsive group were kindled into epilepsy by lithium-pilocarpine 
      method. Expression of GRP78 mRNA and caspase-12 mRNA was detected with reverse 
      transcription-polymerase chain reaction (RT-PCR) method. Expressions of GRP78 and 
      caspase-12 protein were detected with immunohistochemical methods. The neuron 
      apoptosis was observed by TdT-mediated dUTP nick end labeling (TUNEL). RESULTS: 
      (1) Measured by immunohistochemistry the value of OD of GRP78 (0.1480 +/- 0.0164, 
      0.1682 +/- 0.0114, and 0.1540 +/- 0.0102, respectively, 12 h - 48 h points) and 
      caspase-12 (0.1325 +/- 0.0165, 0.1794 +/- 0.0213, 0.1525 +/- 0.0423, and 0.1309 +/- 
      0.0199, respectively, 12 h-72 h points) positive cells in the SC group increased, 
      there was a significant difference compared with NS group (GRP78: 0.1214 +/- 
      0.0147, 0.1272 +/- 0.0177, and 0.1260 +/- 0.0157, respectively, 12 h-72 h points. 
      Caspase-12: 0.1050 +/- 0.0121, 0.1041 +/- 0.0151, 0.1058 +/- 0.0222, and 0.1036 +/- 
      0.0186, respectively, 12 h - 72 h points) (P < 0.01, or P < 0.05). By ED 
      intervention GRP78 (0.1550 +/- 0.0131, 0.1886 +/- 0.0154, and 0.1721 +/- 0.0151, 
      respectively, 12 h - 48 h points) positive cells value of the OD increased as 
      compared with SC group (P < 0.01, or P < 0.05). and caspase-12 (0.1211 +/- 0.0184, 
      0.1545 +/- 0.0205, and 0.1085 +/- 0.0219, respectively, 12 h, 24 h and 72 h points) 
      positive cells value of the A decreased as compared with SC group (P < 0.01, or P 
      < 0.05). (2) Measured by RT-PCR, the expression of GRP78 mRNA and caspase-12 mRNA 
      trend was similar to protein. (3) The TUNEL positive cells in hippocampus CA(1) 
      of SC group (11.41 +/- 2.37) were more than that of NS group after the SC 12 h (P < 
      0.01), reached its highest level at 48 h (28.78 +/- 5.11), after the intervention 
      with edaravone (8.98 +/- 2.22, 13.09 +/- 2.54 and 20.57 +/- 4.89, respectively, 12 h-48 
      h points), TUNEL positive cells showed a significant drop in SC group at 12 h-48 
      h time points (P < 0.01, or P < 0.05), but still significantly higher than that 
      of the NS group (6.22 +/- 1.50, 6.57 +/- 1.61 and 6.72 +/- 1.14, respectively) (P < 
      0.01, or P < 0.05), at the 4 h time point (NS group 6.29 +/- 1.49, SC group 6.61 +/- 
      1.71, ED group 5.75 +/- 1.41) among the three groups, no significant difference in 
      TUNEL positive cells was found (P = 0.759). CONCLUSIONS: The expression of GRP78 
      and caspase-12 increased after SC. Edaravone increased expression of GRP78 and 
      decreased expression of caspase-12 in hippocampus rat with pilocarpine-induced 
      seizures, reduced the number of neuronal apoptosis. These results suggest that 
      edaravone may have protective effect against the hippocampal damage caused by 
      status convulsive.
FAU - Li, Guang-Qian
AU  - Li GQ
AD  - Department of Neurology, The Sixth Hospital Affiliated to Zhejiang University of 
      Traditional Chinese Medicine, Hangzhou Children's Hospital, Hangzhou 310014, 
      China.
FAU - Wang, Hai-Ping
AU  - Wang HP
FAU - Jiang, Chun-Ming
AU  - Jiang CM
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Ke Za Zhi
JT  - Zhonghua er ke za zhi = Chinese journal of pediatrics
JID - 0417427
RN  - 0 (GRP78 protein, rat)
RN  - 0 (Heat-Shock Proteins)
RN  - EC 3.4.22.- (Casp12 protein, rat)
RN  - EC 3.4.22.- (Caspase 12)
RN  - S798V6YJRP (Edaravone)
RN  - T3CHA1B51H (Antipyrine)
SB  - IM
MH  - Animals
MH  - Antipyrine/*analogs & derivatives/pharmacology
MH  - Apoptosis/*drug effects
MH  - Caspase 12/*metabolism
MH  - Edaravone
MH  - Heat-Shock Proteins/*metabolism
MH  - Hippocampus/drug effects/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/*metabolism
EDAT- 2011/03/25 06:00
MHDA- 2011/11/04 06:00
CRDT- 2011/03/25 06:00
PHST- 2011/03/25 06:00 [entrez]
PHST- 2011/03/25 06:00 [pubmed]
PHST- 2011/11/04 06:00 [medline]
PST - ppublish
SO  - Zhonghua Er Ke Za Zhi. 2011 Jan;49(1):53-9.