PMID- 21429432
OWN - NLM
STAT- MEDLINE
DCOM- 20120123
LR  - 20110324
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 46
IP  - 1
DP  - 2011 Jan
TI  - [Importance of aneuploidy screening in preimplantation genetic diagnosis for the 
      couples of chromosome translocation carriers].
PG  - 32-5
AB  - OBJECTIVE: To determine the importance of aneuploidy screening in preimplantation 
      genetic diagnosis for the couples of chromosome translocation carriers. METHODS: 
      To perform 11 prenatal genetic diagnosis (PGD) cycles for 7 couples of chromosome 
      translocation carriers from January 2006 to March 2009 in the Reproductive 
      Medical Center, First Affiliated Hospital of Zhengzhou University. To re-analyze 
      the well-fixed, non-multinuclear and non-debris nuclei using the probes of LSI 
      13, 18, 21, CEPX, CEPY to detect the aneuploidy rate which come from the PGD 
      cycles of the couples of chromosome translocation carriers. The euploid embryo 
      was defined as two fluorescence in situ hybridization (FISH) signals of LSI 13, 
      18, 21 respectively and two signals of CEPX, or one signal of CEPX and one signal 
      of CEPY. The other abnormal signals were defined as aneuploid embryo. RESULTS: 
      (1) A total of 130 nuclei from 11 PGD cycles got the integrated re-FISH signals. 
      Nine hundred and thirty-seven FISH signals were analyzed, including 304 signals 
      from 38 euploid nuclei and the others from 92 aneuploid nuclei. (2) The number of 
      the aneuploid nuclei from grade I, II and III embryo was 20 (22%), 36 (39%), and 
      36 (39%). The number of the euploid nuclei from grade I, II and III embryo was 
      13(34%), 17 (45%), and 8 (21%). There was no significant difference of aneuploidy 
      rate between the embryos form different grades (P > 0.05). However, the rate of 
      aneuploid nucleus from good quality embryos (grade I + grade II) was 60% (59/92). 
      (3) The euploidy rate was 71.4% (30/42) from balanced embryos, while 9.1% (8/88) 
      from unbalanced embryos. There was significant difference between them (chi(2) = 
      53.4, P < 0.05). The rate of aneuploidy from balanced embryos was lower than 
      those from unbalanced embryos (P < 0.05). CONCLUSIONS: Since higher rate of 
      aneuploidy was detected in embryos of the couples of chromosome translocation 
      carriers. It is advisable to recommend the FISH re-analysis for aneuploidy 
      screening to preimplantation genetic diagnosis for the couples of chromosome 
      translocation carriers.
FAU - Li, Gang
AU  - Li G
AD  - Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, China.
FAU - Sun, Ying-pu
AU  - Sun YP
FAU - Jin, Hai-xia
AU  - Jin HX
FAU - Xin, Zhi-min
AU  - Xin ZM
FAU - Dai, Shan-jun
AU  - Dai SJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Female
MH  - Fertilization in Vitro/methods
MH  - *Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Preimplantation Diagnosis/*methods
MH  - Retrospective Studies
MH  - *Translocation, Genetic
EDAT- 2011/03/25 06:00
MHDA- 2012/01/24 06:00
CRDT- 2011/03/25 06:00
PHST- 2011/03/25 06:00 [entrez]
PHST- 2011/03/25 06:00 [pubmed]
PHST- 2012/01/24 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2011 Jan;46(1):32-5.