PMID- 21431632
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20110324
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 730
DP  - 2011
TI  - Fluorescence in situ hybridization methods and troubleshooting applied to fixed 
      cell suspensions.
PG  - 13-31
LID - 10.1007/978-1-61779-074-4_3 [doi]
AB  - A robust procedure for performing fluorescence in situ hybridization (FISH) is 
      described, with tips for troubleshooting. FISH probes are now more reliable and 
      there is a greater range commercially available. FISH is an essential part of the 
      cytogeneticist's repertoire. It remains a powerful, complementary adjunct to 
      karyotype studies, and knowledge of the underlying chromosome abnormalities can 
      be essential for understanding the FISH signal patterns.
FAU - Swansbury, John
AU  - Swansbury J
AD  - Clinical Cytogenetics Laboratory, The Royal Marsden Hospital and The Institute of 
      Cancer Research, Sutton, Surrey, UK. john.swansbury@rmh.nhs.uk
FAU - Min, Toon
AU  - Min T
FAU - Aruliah, Shilani
AU  - Aruliah S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
RN  - 0 (Suspensions)
SB  - IM
MH  - Cells, Immobilized/cytology
MH  - DNA Probes/chemistry/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Denaturation
MH  - Suspensions
EDAT- 2011/03/25 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/03/25 06:00
PHST- 2011/03/25 06:00 [entrez]
PHST- 2011/03/25 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - 10.1007/978-1-61779-074-4_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;730:13-31. doi: 10.1007/978-1-61779-074-4_3.