PMID- 21431638
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20110324
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 730
DP  - 2011
TI  - Cytogenetic methods in chronic lymphocytic leukemia.
PG  - 119-30
LID - 10.1007/978-1-61779-074-4_9 [doi]
AB  - In chronic lymphocytic leukemia of the B-lineage (B-CLL), cytogenetic alterations 
      are highly relevant for prognosis and therapeutic decisions. With conventional 
      techniques, chromosome banding analysis in CLL has been hampered by the low 
      quality of metaphases and low rates of cytogenetic alterations due to a low in 
      vitro proliferation rate of CLL cells. Thus, interphase fluorescence in situ 
      hybridization (FISH) has become the standard technique for cytogenetic analysis 
      in CLL. However, interphase FISH is not able to provide an overview on the whole 
      karyotype. In order to improve chromosome banding analysis in CLL, specific 
      stimulation techniques have been developed. These either use CD40 ligand or 
      oligonucleotides (e.g., CpG) and IL-2 in combination. With the respective 
      techniques, metaphase cultivation is successful in >90% of CLL cases and aberrant 
      karyotypes can be detected in nearly 90% of CLL cases. This has allowed the 
      detection of new clinically relevant subgroups (e.g., complex aberrant karyotype 
      cases) and a more differentiated picture of distinct cytogenetic subtypes, e.g., 
      cases with a 13q deletion. Efforts should continue to define the value of 
      chromosomal banding in CLL focusing as well on the interaction with already 
      established techniques such as interphase FISH or immunophenotyping.
FAU - Haferlach, Claudia
AU  - Haferlach C
AD  - MLL Munich Leukemia Laboratory, Munich, Germany. Claudia.haferlach@mll-online.com
FAU - Bacher, Ulrike
AU  - Bacher U
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Banding
MH  - Cytogenetic Analysis/*methods
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*pathology
MH  - Metaphase/genetics
MH  - Staining and Labeling
EDAT- 2011/03/25 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/03/25 06:00
PHST- 2011/03/25 06:00 [entrez]
PHST- 2011/03/25 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - 10.1007/978-1-61779-074-4_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;730:119-30. doi: 10.1007/978-1-61779-074-4_9.