PMID- 21431640
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20131121
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 730
DP  - 2011
TI  - Metaphase cytogenetic techniques in multiple myeloma.
PG  - 149-58
LID - 10.1007/978-1-61779-074-4_11 [doi]
AB  - Metaphase chromosome studies in multiple myeloma (MM) are performed as part of 
      the diagnostic workup, as surveillance to monitor the therapeutic response, and 
      at relapse to help direct therapy. Unfortunately, the abnormal clones in many 
      patients have a low proliferative activity and therefore, in many cases, the 
      analyzable metaphase cells are derived from normal hematopoiesis. This limitation 
      has been overcome in part by the use of fluorescence in situ hybridization (FISH) 
      of interphase nuclei, which is an important adjunct to metaphase analysis. 
      However, the metaphase karyotype remains the primary cytogenetic tool used at our 
      institute for diagnostic and prognostic purposes. To maximize the possibility of 
      detecting abnormal cells in conventional metaphase studies, we routinely employ 
      at least three different cell harvest techniques on each marrow specimen. These 
      include a direct harvest, a 24 h culture, and either a synchronized or 
      unsynchronized 48 h culture. Recently, fine needle aspirates of solitary 
      plasmacytomas guided by magnetic-resonance imaging have also been shown to 
      provide diagnostic metaphase karyotypes. Regardless of the origin or quality of 
      the specimen, some uninformative cytogenetic results may simply be due to 
      insufficient number and type of cultures being initiated and subsequently 
      examined.
FAU - Sawyer, Jeffrey R
AU  - Sawyer JR
AD  - Department of Pathology, Myeloma Institute for Research and Therapy, University 
      of Arkansas for Medical Sciences, Little Rock, AR, USA. SawyerJeffreyR@uams.edu
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Biopsy, Fine-Needle
MH  - Bone Marrow/drug effects/pathology
MH  - Cell Separation
MH  - Cytogenetic Analysis/*methods
MH  - Humans
MH  - *Metaphase/drug effects/genetics
MH  - Methotrexate/pharmacology
MH  - Multiple Myeloma/*genetics/*pathology
MH  - Plasmacytoma/genetics/pathology
MH  - Specimen Handling
MH  - Time Factors
MH  - Tissue Culture Techniques
EDAT- 2011/03/25 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/03/25 06:00
PHST- 2011/03/25 06:00 [entrez]
PHST- 2011/03/25 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - 10.1007/978-1-61779-074-4_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;730:149-58. doi: 10.1007/978-1-61779-074-4_11.