PMID- 21431737
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20131121
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 714
DP  - 2011
TI  - High-resolution fluorescence in situ hybridization to detect mRNAs in neuronal 
      compartments in vitro and in vivo.
PG  - 103-23
LID - 10.1007/978-1-61779-005-8_7 [doi]
AB  - The localization of specific mRNAs into dendrites and/or axons is an important 
      mechanism to enrich -proteins at their sites of function and influence neuronal 
      development, plasticity, and repair. The fluorescence in situ hybridization 
      (FISH) methods described here have provided high sensitivity and resolution 
      enabling investigation into the mechanism, regulation, and function of mRNA 
      localization in vitro and in vivo. Two methods are described in detail. The first 
      method employs digoxigenin- or fluorophore-conjugated oligonucleotide probes for 
      the detection of localized mRNAs in dendrites, spines, axons, and growth cones of 
      cultured neurons. The second method employs digoxigenin-labeled RNA probes and 
      fluorescence tyramide amplification for the detection of less abundant mRNAs 
      localized to dendrites in vivo. Both methods enable the visualization and 
      quantification of mRNA granules, and changes in their localization in response to 
      various stimuli. The high-resolution FISH technology described here has broader 
      applications beyond the study of mRNA localization. It enables the quantitative 
      analyses of developmental and cell type-specific patterns of gene expression, and 
      how these are modified by physiological signals or during disease states.
FAU - Swanger, Sharon A
AU  - Swanger SA
AD  - Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, 
      USA.
FAU - Bassell, Gary J
AU  - Bassell GJ
FAU - Gross, Christina
AU  - Gross C
LA  - eng
GR  - F31NS063668/NS/NINDS NIH HHS/United States
GR  - HD055835/HD/NICHD NIH HHS/United States
GR  - MH085617/MH/NIMH NIH HHS/United States
GR  - T32GM0860512/GM/NIGMS NIH HHS/United States
GR  - T32NS007480/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (activity regulated cytoskeletal-associated protein)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cytoskeletal Proteins/genetics
MH  - Digoxigenin/metabolism
MH  - Fluorescent Dyes/metabolism
MH  - Hippocampus/cytology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Intracellular Space/metabolism
MH  - Nerve Tissue Proteins/genetics
MH  - Neurons/*cytology/*metabolism
MH  - RNA Probes/genetics/metabolism
MH  - RNA, Messenger/*analysis/genetics/metabolism
MH  - Rats
EDAT- 2011/03/25 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/03/25 06:00
PHST- 2011/03/25 06:00 [entrez]
PHST- 2011/03/25 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - 10.1007/978-1-61779-005-8_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;714:103-23. doi: 10.1007/978-1-61779-005-8_7.