PMID- 21434312
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20161125
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 40
IP  - 1
DP  - 2011 Jan
TI  - [Effect of aflatoxin G1 on the expression of HLA- I molecule in human esophageal 
      epithelial cells].
PG  - 50-2, 56
AB  - OBJECTIVE: To explore the effect of AFG1 on the expression of human leukocyte 
      antigen (HLA-I) molecule in human esophageal epithelial cells. METHOD: Western 
      Blot and RT-PCT analysis was used to explore the effect of AFG1 on the antigen 
      presenting function of primary cultured esophageal epithelial cells. RESULTS: The 
      protein expression of HLA-ABC was significantly decreased in the groups treated 
      with 100, 1000 and 2000 microg/L AFG1 as compared with the solvent control group 
      (P < 0.05). Within the range of 100 - 2000 microg/L AFG1, the protein expression 
      level of HLA-ABC of esophageal epithelial cells decreased gradually, and showed 
      significant negative correlation (r = -0.921, n = 3, P < 0.01). The effect of 
      AFG1 on the expression of HLA-ABC mRNA was further confirmed by RT-PCR analysis. 
      The results showed that after treated with 100 microg/L, 1000 microg/L and 2000 
      microg/L AFG1, the expression of HLA-A mRNA was decreased significantly as 
      compared with the solvent control (P < 0.05), and the expression of HLA-B mRNA 
      was decreased in the 2000 microg/L AFG1 treated group (P < 0.05), while there was 
      no effect on the expression of HLA-C mRNA. CONCLUSION: The expression of HLA- I 
      molecule in human esophageal epithelial cells could be inhibited by AFG1 
      treatment.
FAU - Li, Zengning
AU  - Li Z
AD  - The First Hospital of Hebei Medical University, Shijiazhuang 050031, China. 
      lizengning@126.com
FAU - Cui, Jinfeng
AU  - Cui J
FAU - Zhang, Xianghong
AU  - Zhang X
FAU - Wang, Junling
AU  - Wang J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Aflatoxins)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
RN  - 1DB78J7PUD (aflatoxin G1)
SB  - IM
MH  - Aflatoxins/*pharmacology
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Epithelial Cells/*cytology
MH  - Esophagus/*cytology
MH  - HLA-A Antigens/genetics/metabolism
MH  - HLA-B Antigens/genetics/metabolism
MH  - HLA-C Antigens/genetics/metabolism
MH  - Histocompatibility Antigens Class I/genetics/*metabolism
MH  - Humans
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2011/03/26 06:00
MHDA- 2013/02/22 06:00
CRDT- 2011/03/26 06:00
PHST- 2011/03/26 06:00 [entrez]
PHST- 2011/03/26 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2011 Jan;40(1):50-2, 56.