PMID- 21437195
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Print)
IS  - 1741-427X (Linking)
VI  - 2011
DP  - 2011
TI  - Biochanin a, a phytoestrogenic isoflavone with selective inhibition of 
      phosphodiesterase 4, suppresses ovalbumin-induced airway hyperresponsiveness.
PG  - 635058
LID - 10.1155/2011/635058 [doi]
LID - 635058
AB  - The present study investigated the potential of biochanin A, a phytoestrogenic 
      isoflavone of red clover (Triflolium pratense), for use in treating asthma or 
      chronic obstructive pulmonary disease (COPD). Biochanin A (100 mumol/kg, orally 
      (p.o.)) significantly attenuated airway resistance (R(L)), enhanced pause 
      (P(enh)), and increased lung dynamic compliance (C(dyn)) values induced by 
      methacholine (MCh) in sensitized and challenged mice. It also significantly 
      suppressed an increase in the number of total inflammatory cells, neutrophils, 
      and eosinophils, and levels of cytokines, including interleukin (IL)-2, IL-4, 
      IL-5, and tumor necrosis factor (TNF)-alpha in bronchoalveolar lavage fluid (BALF) of 
      the mice. However, it did not influence interferon (IFN)-gamma levels. Biochanin A 
      (100 mumol/kg, p.o.) also significantly suppressed the total and ovalbumin 
      (OVA)-specific immunoglobulin E (IgE) levels in the serum and BALF, and enhanced 
      the total IgG(2a) level in the serum of these mice. The PDE4(H)/PDE4(L) value of 
      biochanin A was calculated as >35. Biochanin A did not influence 
      xylazine/ketamine-induced anesthesia. Biochanin A (10~30 muM) significantly 
      reduced cumulative OVA (10~100 mug/mL)-induced contractions in the isolated guinea 
      pig trachealis, suggesting that it inhibits degranulation of mast cells. In 
      conclusion, red clover containing biochanin A has the potential for treating 
      allergic asthma and COPD.
FAU - Ko, Wun-Chang
AU  - Ko WC
AD  - Department of Pharmacology, School of Medicine, College of Medicine, Taipei 
      Medical University, Taipei 110, Taiwan.
FAU - Lin, Ling-Hung
AU  - Lin LH
FAU - Shen, Hsin-Yi
AU  - Shen HY
FAU - Lai, Chi-Yin
AU  - Lai CY
FAU - Chen, Chien-Ming
AU  - Chen CM
FAU - Shih, Chung-Hung
AU  - Shih CH
LA  - eng
PT  - Journal Article
DEP - 20110314
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC3062156
EDAT- 2011/03/26 06:00
MHDA- 2011/03/26 06:01
PMCR- 2011/03/14
CRDT- 2011/03/26 06:00
PHST- 2010/10/27 00:00 [received]
PHST- 2011/01/03 00:00 [accepted]
PHST- 2011/03/26 06:00 [entrez]
PHST- 2011/03/26 06:00 [pubmed]
PHST- 2011/03/26 06:01 [medline]
PHST- 2011/03/14 00:00 [pmc-release]
AID - 10.1155/2011/635058 [doi]
PST - ppublish
SO  - Evid Based Complement Alternat Med. 2011;2011:635058. doi: 10.1155/2011/635058. 
      Epub 2011 Mar 14.