PMID- 21438009
OWN - NLM
STAT- MEDLINE
DCOM- 20110719
LR  - 20211020
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 25
IP  - 2
DP  - 2011
TI  - Detection of Staphylococcus aureus with a fluorescence in situ hybridization that 
      does not require lysostaphin.
PG  - 142-7
LID - 10.1002/jcla.20448 [doi]
AB  - To detect with whole-cell fluorescence in situ hybridization (FISH), 
      Staphylococcus aureus is typically permeabilized with lysozyme and lysostaphin. 
      We tested whether it was feasible to detect S. aureus and differentiate it from 
      Staphylococcus epidermidis with lysozyme-only permeabilization. We compared 
      lysozyme permeabilization to S. aureus permeabilized with lysozyme in combination 
      with lysostaphin. It was determined that S. aureus treated with agarose, 
      methanol, and lysozyme could be detected with FISH. The 1  hr protocol is a 
      useful alternative to conventional FISH.
CI  - (c) 2011 Wiley-Liss, Inc.
FAU - Lawson, Thomas S
AU  - Lawson TS
AD  - Faculty of Science, Macquarie University, NSW, Australia. tomxlawson@gmail.com
FAU - Connally, Russell E
AU  - Connally RE
FAU - Iredell, Jonathan R
AU  - Iredell JR
FAU - Vemulpad, Subramanyam
AU  - Vemulpad S
FAU - Piper, James A
AU  - Piper JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.4.24.75 (Lysostaphin)
SB  - IM
MH  - Bacterial Capsules/drug effects
MH  - Cell Membrane Permeability/drug effects
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lysostaphin/*pharmacology
MH  - Microbial Sensitivity Tests
MH  - Muramidase/pharmacology
MH  - Staphylococcus aureus/drug effects/genetics/*isolation & purification
PMC - PMC6647721
EDAT- 2011/03/26 06:00
MHDA- 2011/07/20 06:00
PMCR- 2011/03/15
CRDT- 2011/03/26 06:00
PHST- 2011/03/26 06:00 [entrez]
PHST- 2011/03/26 06:00 [pubmed]
PHST- 2011/07/20 06:00 [medline]
PHST- 2011/03/15 00:00 [pmc-release]
AID - JCLA20448 [pii]
AID - 10.1002/jcla.20448 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2011;25(2):142-7. doi: 10.1002/jcla.20448.