PMID- 21438371
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 12
DP  - 2010 Dec
TI  - [Determination of trans fatty acid C18:1, C18:2 and C18:3 isomers in milk fat by 
      silver ion solid phase extraction-gas chromatography].
PG  - 1168-72
AB  - A method for the determination of trans fatty acids C18:1, C18:2 and C18:3 in 
      milk fat was developed by silver ion solid phase extraction-gas chromatography 
      (Ag+ -SPE/GC). The fatty acid methyl esters derived from total milk fat were 
      loaded onto an Ag+ -SPE cartridge, and then eluted with 9 mL of toluene-hexane 
      (5:95, v/v), 8 mL of toluene-hexane (17:83, v/v), 6 mL of toluene-ethyl acetate 
      (17:83, v/v) and 10 mL of toluene-ethyl acetate (30:70, v/v) in sequence. The 
      fraction from each step was analyzed by GC. The average recoveries of trans fatty 
      acids ranged from 88.4% to 107.2% with the relative standard deviations (RSDs) 
      from 1.2% to 11.9% except trans linolenic acid with the recoveries from 69.9% to 
      101.0% and the RSDs from 11.0% to 18.1%. Comparing with the traditional methods, 
      the developed approach can avoid the interference of cis and saturated fatty 
      acids. The method was successfully applied to analyze dairy products. 
      Satisfactory results were shown.
FAU - Rui, Li
AU  - Rui L
AD  - School of Medical Laboratory Science and School of Life Science, Wenzhou Medical 
      College, Wenzhou 325035, China.
FAU - Xu, Xiaomin
AU  - Xu X
FAU - Li, Yali
AU  - Li Y
FAU - Song, Guoliang
AU  - Song G
FAU - Han, Jianlong
AU  - Han J
FAU - Ren, Yiping
AU  - Ren Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Fats)
RN  - 0 (Trans Fatty Acids)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Chromatography, Gas/*methods
MH  - Fats/*analysis/isolation & purification
MH  - Isomerism
MH  - Milk/*chemistry
MH  - Silver/chemistry
MH  - Solid Phase Extraction/*methods
MH  - Trans Fatty Acids/*analysis/chemistry
EDAT- 2011/03/29 06:00
MHDA- 2012/02/14 06:00
CRDT- 2011/03/29 06:00
PHST- 2011/03/29 06:00 [entrez]
PHST- 2011/03/29 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2010 Dec;28(12):1168-72.