PMID- 21438914
OWN - NLM
STAT- MEDLINE
DCOM- 20110805
LR  - 20110426
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 319
IP  - 1
DP  - 2011 Jun
TI  - Dictyostelium hybrid polyketide synthase, SteelyA, produces 
      4-methyl-5-pentylbenzene-1,3-diol and induces spore maturation.
PG  - 82-7
LID - 10.1111/j.1574-6968.2011.02273.x [doi]
AB  - The genome of Dictyostelium contains two novel hybrid-type polyketide synthases 
      (PKSs) known as 'Steely'; the Steely enzyme is formed by the fusion of type I and 
      type III PKSs. One of these enzymes, SteelyB, is known to be responsible for the 
      production of the stalk cell-inducing factor DIF-1 in vivo. On the other hand, 
      the product(s) and expression pattern of SteelyA are not clearly understood, 
      because there are two different reports associated with the in vitro products of 
      SteelyA and its expression pattern. To solve this problem, we first examined the 
      expression pattern using two different primer sets and found that it was quite 
      similar to that shown in the dictyExpress database. stlA expression peaked at 
      approximately 3 h and declined, but showed a small peak around the end of 
      development. Next, we examined the in vivo product of SteelyA using a stlA null 
      mutant and found that the mutant lacked 4-methyl-5-pentylbenzene-1,3-diol (MPBD). 
      This null mutant showed aberrant, glassy sori, and most of the cells in the sori 
      remained amoeba-like without a cell wall. This defect was restored by adding 200 
      nM of MPBD to the agar. These results indicate that SteelyA produces MPBD in vivo 
      and induces spore maturation.
CI  - (c) 2011 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Narita, Takaaki B
AU  - Narita TB
AD  - Graduate School of Science and Technology, Sophia University, Tokyo, Japan.
FAU - Koide, Kota
AU  - Koide K
FAU - Morita, Naoki
AU  - Morita N
FAU - Saito, Tamao
AU  - Saito T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110411
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (4-methyl-5-pentylbenzene-1,3-diol)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Resorcinols)
RN  - 79956-01-7 (Polyketide Synthases)
SB  - IM
MH  - Dictyostelium/*enzymology/genetics/growth & development/metabolism
MH  - Polyketide Synthases/genetics/*metabolism
MH  - Protozoan Proteins/genetics/*metabolism
MH  - Resorcinols/*metabolism
MH  - Spores, Protozoan/enzymology/genetics/*growth & development/metabolism
EDAT- 2011/03/29 06:00
MHDA- 2011/08/06 06:00
CRDT- 2011/03/29 06:00
PHST- 2011/03/29 06:00 [entrez]
PHST- 2011/03/29 06:00 [pubmed]
PHST- 2011/08/06 06:00 [medline]
AID - 10.1111/j.1574-6968.2011.02273.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2011 Jun;319(1):82-7. doi: 10.1111/j.1574-6968.2011.02273.x. 
      Epub 2011 Apr 11.