PMID- 21440019
OWN - NLM
STAT- MEDLINE
DCOM- 20110908
LR  - 20211020
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 158
IP  - 1-2
DP  - 2011 Jun
TI  - Identification of non-essential regions in nucleocapsid protein of porcine 
      reproductive and respiratory syndrome virus for replication in cell culture.
PG  - 62-71
LID - 10.1016/j.virusres.2011.03.011 [doi]
AB  - Nucleocapsid (N) protein of porcine reproductive and respiratory syndrome virus 
      (PRRSV) plays a central role in virus replication. In this study, serial N- and 
      C-terminal truncations of N protein were performed in the context of type 2 PRRSV 
      infectious cDNA clone, and our results revealed that a stretch of inter-genotypic 
      variable N terminal residues aa 5-13 ((5)NGKQQKKK(13)K) and the last four 
      inter-genotypic variable aa residues ((120)SPS(123)A) at the C terminus of N 
      protein were dispensable for type 2 PRRSV infectivity. All the recovered deletion 
      mutant viruses had spontaneous mutations in the N coding region, including 
      substitution, deletion and insertion. We re-engineered the additional internal 
      deletion with or without the original C-terminal deletion back into wild-type 
      APRRS and found that the internal domain spanning the inter-genotypic variable 
      residues 39-42 ((39)KGP(42)G) and conserved residues 48-52 ((48)KNPE(52)K), 
      respectively, were dispensable for type 2 PRRSV viability. These results 
      demonstrated that N protein contains non-essential regions for virus viability in 
      cell culture. Such dispensable regions could be utilized as insertion site for 
      foreign tag expression and the rescued viruses could be the candidates for marker 
      vaccine.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Tan, Feifei
AU  - Tan F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Shanghai 200241, 
      China.
FAU - Wei, Zuzhang
AU  - Wei Z
FAU - Li, Yanhua
AU  - Li Y
FAU - Zhang, Rong
AU  - Zhang R
FAU - Zhuang, Jinshan
AU  - Zhuang J
FAU - Sun, Zhi
AU  - Sun Z
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110331
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Cell Culture Techniques
MH  - DNA Mutational Analysis
MH  - Microbial Viability
MH  - Mutagenesis, Insertional
MH  - Nucleocapsid Proteins/*genetics/*metabolism
MH  - Point Mutation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*growth & 
      development
MH  - Sequence Deletion
MH  - *Virus Replication
PMC - PMC7114398
EDAT- 2011/03/29 06:00
MHDA- 2011/09/09 06:00
PMCR- 2011/03/24
CRDT- 2011/03/29 06:00
PHST- 2010/12/20 00:00 [received]
PHST- 2011/03/12 00:00 [revised]
PHST- 2011/03/14 00:00 [accepted]
PHST- 2011/03/29 06:00 [entrez]
PHST- 2011/03/29 06:00 [pubmed]
PHST- 2011/09/09 06:00 [medline]
PHST- 2011/03/24 00:00 [pmc-release]
AID - S0168-1702(11)00101-8 [pii]
AID - 10.1016/j.virusres.2011.03.011 [doi]
PST - ppublish
SO  - Virus Res. 2011 Jun;158(1-2):62-71. doi: 10.1016/j.virusres.2011.03.011. Epub 
      2011 Mar 31.