PMID- 21442765
OWN - NLM
STAT- MEDLINE
DCOM- 20120919
LR  - 20211020
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Print)
IS  - 1932-6254 (Linking)
VI  - 6
IP  - 3
DP  - 2012 Mar
TI  - Mannitol-containing macroporous calcium phosphate cement encapsulating human 
      umbilical cord stem cells.
PG  - 214-24
LID - 10.1002/term.419 [doi]
AB  - Stem cell-based tissue engineering offers immense promise for bone regeneration. 
      The objective of this study was to develop a self-setting, mannitol-containing 
      calcium phosphate cement (CPC) encapsulating human umbilical cord mesenchymal 
      stem cells (hUCMSCs) for bone tissue engineering. hUCMSCs could be an 
      inexhaustible and low-cost alternative to the gold-standard bone marrow MSCs, 
      which require an invasive procedure to harvest. hUCMSCs were encapsulated in 
      alginate beads and mixed into the CPC paste. Water-soluble mannitol porogen was 
      incorporated into CPC to create macropores. The porosity was increased from 49% 
      for the hUCMSC-encapsulating CPC to 64% after adding mannitol and 
      absorbable-fibres (p < 0.05). Flexural strength of the construct was increased 
      from 0.3 MPa to 2.0 MPa via fibres. Live cell percentage was > 80% for all 
      constructs. The ALP and OC gene expressions were low at 1 day and greatly 
      increased at 14 days. The constructs that contained mannitol had significantly 
      higher ALP and OC expressions than that without mannitol. ALP activity of hUCMSCs 
      inside CPC with mannitol and fibre was significantly higher than that without 
      mannitol. At 14 days, mineralization by the encapsulated hUCMSCs was eight-fold 
      higher than that at 1 day. In conclusion, a novel mannitol-containing porous 
      CPC-hUCMSC construct was developed for bone tissue engineering. Its advantages 
      include cell delivery inside a load-bearing CPC that has injectable and in situ 
      setting capabilities. hUCMSCs inside CPC had good viability and successfully 
      osteodifferentiated. The self-setting and strong hUCMSC-encapsulating CPC 
      scaffold is promising for bone tissue engineering in a wide range of orthopaedic 
      and craniofacial applications.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Tang, Minghui
AU  - Tang M
AD  - Department of Endodontics, Prosthodontics and Operative Dentistry, University of 
      Maryland Dental School, Baltimore, MD 21201, USA.
FAU - Weir, Michael D
AU  - Weir MD
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190-09/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-10/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-07A1S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110327
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 3OWL53L36A (Mannitol)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Bone Cements/*pharmacology
MH  - Calcification, Physiologic/drug effects
MH  - Calcium Phosphates/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Immobilized/cytology/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mannitol/*pharmacology
MH  - Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - Osteogenesis/drug effects
MH  - Porosity/drug effects
MH  - Stem Cells/*cytology/*drug effects/enzymology
MH  - Umbilical Cord/*cytology
PMC - PMC3144283
MID - NIHMS287815
EDAT- 2011/03/29 06:00
MHDA- 2012/09/20 06:00
PMCR- 2013/03/01
CRDT- 2011/03/29 06:00
PHST- 2010/09/16 00:00 [received]
PHST- 2011/02/17 00:00 [accepted]
PHST- 2011/03/29 06:00 [entrez]
PHST- 2011/03/29 06:00 [pubmed]
PHST- 2012/09/20 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - 10.1002/term.419 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2012 Mar;6(3):214-24. doi: 10.1002/term.419. Epub 2011 
      Mar 27.