PMID- 21444253
OWN - NLM
STAT- MEDLINE
DCOM- 20110802
LR  - 20161125
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 13-14
DP  - 2011 Apr 15
TI  - Highly sensitive profiling assay of acidic plant hormones using a novel mass 
      probe by capillary electrophoresis-time of flight-mass spectrometry.
PG  - 938-44
LID - 10.1016/j.jchromb.2011.03.003 [doi]
AB  - Plant hormones play crucial roles in plant growth and development. However, up to 
      date, identification and quantification of acidic plant hormones with trace 
      amount in complicated plant matrix is still a challenge. In current study, we 
      developed a high sensitive assay for the determination of acidic plant hormones 
      in rice by combining capillary electrophoresis and electrospray ionization-time 
      of flight-mass spectrometry (CE-ESI-TOF-MS). To improve the detection sensitivity 
      of acidic plant hormones, 3-bromoactonyltrimethylammonium bromide (BTA) was 
      synthesized as a new mass probe, which can react efficiently with acidic plant 
      hormones in acetonitrile containing triethylamine (TEA). The positively charged 
      BTA-derivatives were separated by CE using amino-coated capillary, which provided 
      a reversed electroosmotic flow (EOF) at low pH, as well as reduced the adsorption 
      of BTA-derivatives on the inner wall of capillary. Using the CE-ESI-TOF-MS method 
      developed in current study, 15 acidic plant hormones, including 10 gibberellins 
      (GAs), were identified and quantified with good linearities from 1.3 to 850 ng/mL 
      with linear coefficient R(2) values of >0.99. The limits of detection (LODs) were 
      in the range of 0.34-4.59 ng/mL. Recoveries of compounds from spiked beverage 
      samples ranged from 84.6 to 112.2%. And a good reproducibility was obtained by 
      evaluating the intra and inter-day precisions with relative standard deviations 
      (RSDs) less than 6.7 and 9.9%, respectively.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chen, Ming-Luan
AU  - Chen ML
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, China.
FAU - Huang, Yun-Qing
AU  - Huang YQ
FAU - Liu, Jia-Qi
AU  - Liu JQ
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110310
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Formates)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0YIW783RG1 (formic acid)
RN  - 16048-36-5 (3-bromoacetonyl trimethylammonium)
SB  - IM
MH  - Adsorption
MH  - Electrophoresis, Capillary/*methods
MH  - Formates/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Mass Spectrometry/*methods
MH  - Oryza/chemistry
MH  - Plant Growth Regulators/*analysis/chemistry
MH  - Quaternary Ammonium Compounds/chemistry
MH  - Regression Analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2011/03/30 06:00
MHDA- 2011/08/04 06:00
CRDT- 2011/03/30 06:00
PHST- 2010/12/17 00:00 [received]
PHST- 2011/02/26 00:00 [revised]
PHST- 2011/03/02 00:00 [accepted]
PHST- 2011/03/30 06:00 [entrez]
PHST- 2011/03/30 06:00 [pubmed]
PHST- 2011/08/04 06:00 [medline]
AID - S1570-0232(11)00149-8 [pii]
AID - 10.1016/j.jchromb.2011.03.003 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15;879(13-14):938-44. 
      doi: 10.1016/j.jchromb.2011.03.003. Epub 2011 Mar 10.