PMID- 21446514
OWN - NLM
STAT- MEDLINE
DCOM- 20110511
LR  - 20190715
IS  - 1533-4880 (Print)
IS  - 1533-4880 (Linking)
VI  - 11
IP  - 1
DP  - 2011 Jan
TI  - Mass imaging of iron oxide nanoparticles inside cells for in vitro cytotoxicity.
PG  - 638-41
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging analysis was 
      performed on murine macrophage cells treated with various concentrations of iron 
      oxide (Fe3O4) nanoparticles, which are used as MRI contrast agents. First, murine 
      macrophage cells were seeded on a slide glass for 24 hrs and treated with varying 
      concentrations of Fe3O4 nanoparticles for 24 hrs. To expose a cross section of 
      each cell and obtain a distribution of the nanoparticles inside the cells, the 
      cells were sputtered using Bi ions after which the cross section of each cell was 
      scanned and imaged using the focused cluster ion beam with a spatial resolution 
      of 300 nm. Fe3O4 nanoparticles were found mainly in the cytoplasm region of the 
      cells, not in the nucleus region of cells, suggesting that the uptake of the 
      Fe3O4 nanoparticles were into the cytoplasm of cell, not into the nucleus of 
      cell. Based on these observations, our protocol using mass imaging analysis would 
      be a useful addition to the study of in vitro nanoparticle cytotoxicity.
FAU - Shon, Hyun Kyong
AU  - Shon HK
AD  - Center for Nano-Bio Convergence Research, Korea Research Institute of Standards 
      and Science, Daejeon 305-600, Korea.
FAU - Park, Jungsin
AU  - Park J
FAU - Choi, Inhong
AU  - Choi I
FAU - Park, Hyun Min
AU  - Park HM
FAU - Moon, Dae Won
AU  - Moon DW
FAU - Lee, Tae Geol
AU  - Lee TG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nanosci Nanotechnol
JT  - Journal of nanoscience and nanotechnology
JID - 101088195
RN  - 0 (Ferric Compounds)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cell Survival
MH  - Cytological Techniques/methods
MH  - Cytoplasm/metabolism
MH  - Ferric Compounds/*analysis/metabolism/toxicity
MH  - Macrophages/*chemistry/cytology/drug effects/metabolism
MH  - Metal Nanoparticles/*analysis/chemistry/toxicity
MH  - Mice
MH  - Spectrometry, Mass, Secondary Ion/methods
MH  - Toxicity Tests
EDAT- 2011/03/31 06:00
MHDA- 2011/05/12 06:00
CRDT- 2011/03/31 06:00
PHST- 2011/03/31 06:00 [entrez]
PHST- 2011/03/31 06:00 [pubmed]
PHST- 2011/05/12 06:00 [medline]
AID - 10.1166/jnn.2011.3273 [doi]
PST - ppublish
SO  - J Nanosci Nanotechnol. 2011 Jan;11(1):638-41. doi: 10.1166/jnn.2011.3273.