PMID- 214473
OWN - NLM
STAT- MEDLINE
DCOM- 19790223
LR  - 20131121
IS  - 0095-1544 (Print)
IS  - 0095-1544 (Linking)
VI  - 4
IP  - 5
DP  - 1978 Oct
TI  - Cyclic AMP-dependent regulation of ornithine decarboxylase activity in Chinese 
      hamster ovary cells maintained with a salts/glucose medium.
PG  - 375-87
AB  - Ornithine decarboxylase activity (ODC) increased about 7 fold 6--8 h following 
      10mM asparagine (ASN) addition to confluent cultures that had been previously 
      serum deprived and then placed in a salts/glucose medium. Optimal concentrations 
      of dibutyryl cAMP (dB cAMP) when incubated with the ASN caused up to a 50 fold 
      increase in the activity of this enzyme after 7--8 h. The enhancement of ODC 
      activity by ASN and dB cAMP was not sensitive to continuous (0--7 h) treatment 
      with actinomycin D but similar treatment with cycloheximide depressed enzyme 
      activity 40--60%. The synergistic stimulation of ODC activity by dB cAMP added 
      with ASN was dose dependent and the dB cAMP stimulation of ODC activity displayed 
      an absolute requirement for ASN when cells were maintained in the salts/glucose 
      medium. The addition of dB cAMP always further enhanced ODC activity above the 
      levels produced by addition of various levels of ASN (1 to 40mM) to the 
      salts/glucose medium. Other agents which elevated cAMP levels such as 
      1-methyl-3-isobutylxanthine (IBMX) also enhanced ODC activity when administered 
      with ASN. Additionally, treatment with sodium butyrate at concentrations ranging 
      from 0.001mM to 5.0mM did not elevate ODC activity above the activity obtained 
      with ASN alone. Addition of dB cAMP at various times after placing cells in 
      salts/glucose medium with ASN further stimulated ODC activity only when added 
      during the first 3-4 h. These results demonstrate the involvement of cAMP in the 
      ASN mediated stimulation of ODC activity using cells maintained in a 
      salts/glucose medium.
FAU - Costa, M
AU  - Costa M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cyclic Nucleotide Res
JT  - Journal of cyclic nucleotide research
JID - 7511483
RN  - 0 (Culture Media)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 7006-34-0 (Asparagine)
RN  - 98600C0908 (Cycloheximide)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
SB  - IM
MH  - Animals
MH  - Asparagine/pharmacology
MH  - Bucladesine/pharmacology
MH  - Carboxy-Lyases/*metabolism
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Cricetulus
MH  - Culture Media
MH  - Cyclic AMP/*physiology
MH  - Cycloheximide/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Female
MH  - Ornithine Decarboxylase/*metabolism
MH  - Ovary/enzymology
MH  - Stimulation, Chemical
MH  - Time Factors
EDAT- 1978/10/01 00:00
MHDA- 1978/10/01 00:01
CRDT- 1978/10/01 00:00
PHST- 1978/10/01 00:00 [pubmed]
PHST- 1978/10/01 00:01 [medline]
PHST- 1978/10/01 00:00 [entrez]
PST - ppublish
SO  - J Cyclic Nucleotide Res. 1978 Oct;4(5):375-87.