PMID- 21447635
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20240417
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 152
IP  - 6
DP  - 2011 Jun
TI  - Separate critical periods exist for testosterone-induced differentiation of the 
      brain and genitals in sheep.
PG  - 2409-15
LID - 10.1210/en.2010-1445 [doi]
AB  - Sheep exposed to testosterone during a critical period from gestational day (GD) 
      30 to GD 90 develop masculine genitals and an enlarged male-typical ovine 
      sexually dimorphic nucleus of the preoptic area (oSDN). The present study tested 
      the hypothesis that separate critical periods exist for masculinization of these 
      two anatomical end points. Pregnant ewes were treated with testosterone 
      propionate (TP) either from GD 30 to GD 60 (early TP) or GD 60 to GD 90 (late 
      TP). Control (C) pregnant ewes were treated with corn oil. Fetuses were delivered 
      at GD 135 and the volume of the oSDN was measured. Early TP females possessed a 
      penis and a scrotum devoid of testes, whereas late TP and C females had normal 
      female genitals. Neither period of TP exposure grossly affected the genitals of 
      male fetuses. Despite masculinized genitals, the mean volume of the oSDN in early 
      TP females (0.32 +/- 0.06 mm(3)) was not different from C females (0.24 +/- 0.02 mm(3)) 
      but was significantly enlarged in late TP females (0.49 +/- 0.04 mm(3); P < 0.05 vs. 
      C) when the genitals appeared normal. In contrast, the volume of the oSDN in late 
      TP males (0.51 +/- 0.02 mm(3)) was not different from C males (0.51 +/- 0.04 mm(3)) but 
      was significantly smaller in the early TP males (0.35 +/- 0.04 mm(3); P < 0.05 vs. 
      C). These results demonstrate that the prenatal critical period for 
      androgen-dependent differentiation of the oSDN occurs later than, and can be 
      separated temporally from, the period for development of masculine genitals.
FAU - Roselli, Charles E
AU  - Roselli CE
AD  - Department of Physiology and Pharmacology L334, Oregon Health and Science 
      University, Portland, Oregon 97239-3098, USA. rosellic@ohsu.edu
FAU - Estill, Charles T
AU  - Estill CT
FAU - Stadelman, Henry L
AU  - Stadelman HL
FAU - Meaker, Mary
AU  - Meaker M
FAU - Stormshak, Fred
AU  - Stormshak F
LA  - eng
GR  - R01 RR014270/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110329
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Animals
MH  - Brain/*embryology/*metabolism
MH  - Critical Period, Psychological
MH  - Female
MH  - Genitalia/*embryology/*metabolism
MH  - Male
MH  - Pregnancy
MH  - Preoptic Area/embryology/metabolism
MH  - *Sex Differentiation
MH  - Sheep/embryology/*physiology
MH  - Testosterone/*metabolism
PMC - PMC3206706
EDAT- 2011/03/31 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/06/01
CRDT- 2011/03/31 06:00
PHST- 2011/03/31 06:00 [entrez]
PHST- 2011/03/31 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/06/01 00:00 [pmc-release]
AID - en.2010-1445 [pii]
AID - EN-10-1445 [pii]
AID - 10.1210/en.2010-1445 [doi]
PST - ppublish
SO  - Endocrinology. 2011 Jun;152(6):2409-15. doi: 10.1210/en.2010-1445. Epub 2011 Mar 
      29.