PMID- 21448164
OWN - NLM
STAT- MEDLINE
DCOM- 20110621
LR  - 20231115
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Print)
IS  - 0007-0920 (Linking)
VI  - 104
IP  - 8
DP  - 2011 Apr 12
TI  - CpG-island methylation study of liver fluke-related cholangiocarcinoma.
PG  - 1313-8
LID - 10.1038/bjc.2011.102 [doi]
AB  - BACKGROUND: Genetic changes have been widely reported in association with 
      cholangiocarcinoma (CCA), while epigenetic changes are poorly characterised. We 
      aimed to further evaluate CpG-island hypermethylation in CCA at candidate loci, 
      which may have potential as diagnostic or prognostic biomarkers. METHODS: We 
      analysed methylation of 26 CpG-islands in 102 liver fluke related-CCA and 29 
      adjacent normal samples using methylation-specific PCR (MSP). Methylation of 
      interest loci was confirmed using pyrosequencing and/or combined bisulfite 
      restriction analysis, and protein expression by immunohistochemistry. RESULTS: A 
      number of CpG-islands (OPCML, SFRP1, HIC1, PTEN and DcR1) showed frequency of 
      hypermethylation in >28% of CCA, but not adjacent normal tissues. The results 
      showed that 91% of CCA were methylated in at least one CpG-island. The OPCML was 
      the most frequently methylated locus (72.5%) and was more frequently methylated 
      in less differentiated CCA. Patients with methylated DcR1 had significantly 
      longer overall survival (Median; 41.7 vs 21.7 weeks, P=0.027). Low-protein 
      expression was found in >70% of CCA with methylation of OPCML or DcR1. 
      CONCLUSION: Aberrant hypermethylation of certain loci is a common event in liver 
      fluke-related CCA and may potentially contribute to cholangiocarcinogenesis. The 
      OPCML and DcR1 might serve as methylation biomarkers in CCA that can be readily 
      examined by MSP.
FAU - Sriraksa, R
AU  - Sriraksa R
AD  - Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Zeller, C
AU  - Zeller C
FAU - El-Bahrawy, M A
AU  - El-Bahrawy MA
FAU - Dai, W
AU  - Dai W
FAU - Daduang, J
AU  - Daduang J
FAU - Jearanaikoon, P
AU  - Jearanaikoon P
FAU - Chau-In, S
AU  - Chau-In S
FAU - Brown, R
AU  - Brown R
FAU - Limpaiboon, T
AU  - Limpaiboon T
LA  - eng
GR  - 13086/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110329
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
SB  - IM
MH  - Aged
MH  - Animals
MH  - Bile Duct Neoplasms/*etiology/*genetics
MH  - *Bile Ducts, Intrahepatic/pathology
MH  - Case-Control Studies
MH  - Cholangiocarcinoma/*etiology/*genetics
MH  - CpG Islands/*genetics
MH  - *DNA Methylation
MH  - Epigenomics
MH  - Fasciola hepatica/physiology
MH  - Fascioliasis/*complications
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
PMC - PMC3078588
EDAT- 2011/03/31 06:00
MHDA- 2011/06/22 06:00
PMCR- 2012/04/12
CRDT- 2011/03/31 06:00
PHST- 2011/03/31 06:00 [entrez]
PHST- 2011/03/31 06:00 [pubmed]
PHST- 2011/06/22 06:00 [medline]
PHST- 2012/04/12 00:00 [pmc-release]
AID - bjc2011102 [pii]
AID - 10.1038/bjc.2011.102 [doi]
PST - ppublish
SO  - Br J Cancer. 2011 Apr 12;104(8):1313-8. doi: 10.1038/bjc.2011.102. Epub 2011 Mar 
      29.