PMID- 21450636
OWN - NLM
STAT- MEDLINE
DCOM- 20120206
LR  - 20151119
IS  - 1741-8275 (Electronic)
IS  - 1741-8267 (Linking)
VI  - 18
IP  - 4
DP  - 2011 Aug
TI  - Stepwise increase of angiopoietin-2 serum levels is related to haemodynamic and 
      functional impairment in stable chronic heart failure.
PG  - 607-14
LID - 10.1177/1741826710389410 [doi]
AB  - BACKGROUND: A reciprocal link between inflammation, oxidative/nitrosative stress, 
      and endothelial dysfunction has been postulated in chronic heart failure (CHF). 
      The endothelial repair mechanisms involved remain to be determined. Our aim was 
      to investigate whether there are detectable signs of ongoing angiogenesis in 
      serum of CHF patients and to evaluate the correlation with indexes of 
      haemodynamic and functional impairment. METHODS AND RESULTS: Enzyme-linked 
      immunosorbent assay tests were used to quantify angiogenin, angiopoietin-1, 
      angiopoietin-2, vascular endothelial growth factor, Tie-2, and brain natriuretic 
      peptide in serum of 87 patients with CHF of increasing severity according to New 
      York Heart Association (NYHA; class I, n = 8; II, n = 45; and III, n = 34) and in 
      14 healthy subjects matched for age and sex. Angiogenin, angiopoietin-2, and 
      Tie-2 were significantly increased in CHF of increasing severity (Kruskal-Wallis: 
      p = 0.0004, p < 0.0001, and p = 0.017, respectively). Angiopoietin-2 was 
      inversely correlated with the 6-min walking test (r = -0.65, p < 0.0001), peak 
      oxygen consumption (VO(2max); r = -0.57, p = 0.0002), and deceleration time 
      (r = -0.61, p < 0.0001). Multiple regression analysis showed that angiopoietin-2 
      was mainly associated with VO(2max) (p = 0.018). The angiopoietin-2 area under 
      the receiver operating characteristic curve for CHF diagnosis was 0.94 (95% CI 
      0.88-0.99; p < 0.001). CONCLUSIONS: These data demonstrate that angiopoietin-2 
      and selected serum markers of angiogenesis progressively increase with 
      haemodynamic and functional decline in CHF.
FAU - Eleuteri, Ermanno
AU  - Eleuteri E
AD  - Divisione di Cardiologia Riabilitativa, Fondazione Salvatore Maugeri, IRCCS, 
      Istituto Scientifico di Veruno, Veruno (NO), Italy.
FAU - Di Stefano, Antonino
AU  - Di Stefano A
FAU - Tarro Genta, Franco
AU  - Tarro Genta F
FAU - Vicari, Chiara
AU  - Vicari C
FAU - Gnemmi, Isabella
AU  - Gnemmi I
FAU - Colombo, Marilena
AU  - Colombo M
FAU - Mezzani, Alessandro
AU  - Mezzani A
FAU - Giannuzzi, Pantaleo
AU  - Giannuzzi P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110211
PL  - England
TA  - Eur J Cardiovasc Prev Rehabil
JT  - European journal of cardiovascular prevention and rehabilitation : official 
      journal of the European Society of Cardiology, Working Groups on Epidemiology & 
      Prevention and Cardiac Rehabilitation and Exercise Physiology
JID - 101192000
RN  - 0 (ANGPT1 protein, human)
RN  - 0 (Angiopoietin-1)
RN  - 0 (Angiopoietin-2)
RN  - 0 (Biomarkers)
RN  - 0 (Peptide Fragments)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (pro-brain natriuretic peptide (1-76))
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - EC 2.7.10.1 (Receptor, TIE-2)
RN  - EC 3.1.27.- (angiogenin)
RN  - EC 3.1.27.5 (Ribonuclease, Pancreatic)
SB  - IM
MH  - Aged
MH  - Analysis of Variance
MH  - Angiopoietin-1/blood
MH  - Angiopoietin-2/*blood
MH  - Biomarkers/blood
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Heart Failure/*blood/*physiopathology
MH  - *Hemodynamics
MH  - Humans
MH  - Italy
MH  - Male
MH  - Middle Aged
MH  - Natriuretic Peptide, Brain/blood
MH  - *Neovascularization, Physiologic
MH  - Oxygen Consumption
MH  - Peptide Fragments/blood
MH  - Receptor, TIE-2/blood
MH  - Regression Analysis
MH  - Ribonuclease, Pancreatic/blood
MH  - Severity of Illness Index
MH  - Stroke Volume
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/blood
MH  - Ventricular Function, Left
EDAT- 2011/04/01 06:00
MHDA- 2012/02/07 06:00
CRDT- 2011/04/01 06:00
PHST- 2011/04/01 06:00 [entrez]
PHST- 2011/04/01 06:00 [pubmed]
PHST- 2012/02/07 06:00 [medline]
AID - 1741826710389410 [pii]
AID - 10.1177/1741826710389410 [doi]
PST - ppublish
SO  - Eur J Cardiovasc Prev Rehabil. 2011 Aug;18(4):607-14. doi: 
      10.1177/1741826710389410. Epub 2011 Feb 11.