PMID- 21451793
OWN - NLM
STAT- MEDLINE
DCOM- 20110812
LR  - 20121115
IS  - 1842-1121 (Electronic)
IS  - 1841-8724 (Linking)
VI  - 20
IP  - 1
DP  - 2011 Mar
TI  - Silencing of the MMP-3 gene by siRNA transfection in gastric cancer AGS cells.
PG  - 19-26
AB  - BACKGROUND: The gastric epithelium is continuously exposed to toxic reactive 
      oxygen species and matrix metalloproteinases (MMPs) are the enzymes known for 
      their roles in the invasion of tumor cells. Here, we report the suppression of 
      matrix metalloproteinase 3 (MMP-3) in gastric cancer cell line AGS by small 
      interfering RNA (siRNA) transfection and its potential role in gastric cancers. 
      METHODS: Oxidative stress in the cell lines was assessed following H2O2 exposure 
      using an oxidative stress marker, 2,7-dichlorofluorescein diacetate (DCFDA). 
      Transfection of cells with small interfering RNA specific to MMP-3, Transwell 
      invasion assay, quantitative reverse transcriptase polymerase chain reaction 
      analysis, and over-expression of MMP-3 were used to determine the potential role 
      of MMP-3 gene in gastric cancers. RESULTS: The silencing of the MMP-3 gene 
      resulted in a decrease of invading AGS while the over-expression of it caused an 
      increase in the invading cells compared to the untreated control cells. Moreover, 
      it also caused a 4.1 fold increase in matrix metalloproteinase 10 (MMP-10) and a 
      7.4 fold decrease in matrix metalloproteinase 15 (MMP-15) mRNA expression levels. 
      CONCLUSIONS: Our results show that the silencing of the MMP-3 gene decreases the 
      number of invading AGS cells and additionally, affects the expressions of MMP-10 
      and MMP-15, suggesting that targeting the MMP-3 gene in gastric cancers might be 
      a therapeutic approach due to its effects on the invasion of AGS cells and the 
      expression of the MMP-15 gene.
FAU - Gencer, Salih
AU  - Gencer S
AD  - Department of Genetics and Bioengineering, Faculty of Engineering, Fatih 
      University, Buyukcekmece, Istambul, Turkey.
FAU - Cebeci, Anil
AU  - Cebeci A
FAU - Irmak-Yazicioglu, Meliha Burcu
AU  - Irmak-Yazicioglu MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Romania
TA  - J Gastrointestin Liver Dis
JT  - Journal of gastrointestinal and liver diseases : JGLD
JID - 101272825
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 15)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.22 (Matrix Metalloproteinase 10)
SB  - IM
MH  - Cell Line, Tumor
MH  - Gene Silencing
MH  - Humans
MH  - Matrix Metalloproteinase 10/genetics
MH  - Matrix Metalloproteinase 15/genetics
MH  - Matrix Metalloproteinase 3/genetics/*physiology
MH  - Matrix Metalloproteinase Inhibitors
MH  - Neoplasm Invasiveness
MH  - Oxidative Stress
MH  - RNA, Small Interfering/*genetics
MH  - Stomach Neoplasms/metabolism/*pathology
MH  - Transfection
EDAT- 2011/04/01 06:00
MHDA- 2011/08/13 06:00
CRDT- 2011/04/01 06:00
PHST- 2011/04/01 06:00 [entrez]
PHST- 2011/04/01 06:00 [pubmed]
PHST- 2011/08/13 06:00 [medline]
AID - 5 [pii]
PST - ppublish
SO  - J Gastrointestin Liver Dis. 2011 Mar;20(1):19-26.