PMID- 21451936
OWN - NLM
STAT- MEDLINE
DCOM- 20120921
LR  - 20211020
IS  - 1573-7403 (Electronic)
IS  - 1386-341X (Linking)
VI  - 15
IP  - 2
DP  - 2012 Jun
TI  - Colonic neoplasia in acromegaly: increased proliferation or deceased apoptosis?
PG  - 166-73
LID - 10.1007/s11102-011-0300-9 [doi]
AB  - Patients with acromegaly have higher prevalence of colorectal neoplasms. The 
      pathogenetic mechanism is still unclear and may be related to sustained increase 
      in serum GH-IGF1. We aimed to evaluate the proliferative and apoptotic markers in 
      samples of colonic mucosa obtained during screening colonoscopic biopsy from 
      patients with acromegaly and study their relationship to serum IGF-1 and GH 
      levels. The study subjects included 32 patients with acromegaly (4 female), 10 
      healthy controls (irritable bowel syndrome) and 10 positive controls 
      (non-acromegalic colonic adenocarcinoma). Patients with acromegaly were divided 
      into two groups, active disease (AD) and disease in remission (AR). Two biopsies 
      each were obtained during colonoscopy from the right colon, transverse colon and 
      rectosigmoid region. All the polyps were biopsied and subjected to 
      histopathological examination. Immunohistochemistry for proliferation marker 
      (Ki-67) and apoptotic markers (caspase-3 and TdT-Mediated dUTP Nick-End Labeling 
      (TUNEL) was carried out in the histopathological samples. Indices of 
      proliferation were significantly different in patients with acromegaly as 
      compared to healthy controls. The mean Ki-67 positivity was 45.1 +/- 17.7% in AD 
      and 45.6 +/- 23.1% in AR, as compared to 10 +/- 5% in healthy controls. While none of 
      the healthy controls had Ki-67 positivity beyond the lower third of crypts, among 
      patients with acromegaly 12/32 (37.5%) had mid-third positivity (P = 0.000) and 
      15/32 (46.8%) had full length of crypt positively (P = 0.00). Immunostaining for 
      caspase-3 was negative in patients with acromegaly and healthy controls. TUNEL 
      was strongly positive in patients with colonic adenocarcinoma but not in healthy 
      controls and patients with acromegaly. IGF-1 levels were higher in those with 
      Ki-67 positivity in the superficial mucosa. Patients with acromegaly have 
      increased proliferation of colonic epithelial cells. Elevated levels of serum 
      IGF1 are associated with increase proliferation in the superficial crypt cells.
FAU - Dutta, Pinaki
AU  - Dutta P
AD  - Departments of Endocinology, Postgraduate Institute of Medical Education and 
      Research, Chandigarh, 160012, India. pinaki_dutta@hotmail.com
FAU - Bhansali, Anil
AU  - Bhansali A
FAU - Vaiphei, Kim
AU  - Vaiphei K
FAU - Dutta, Usha
AU  - Dutta U
FAU - Ravi Kumar, P
AU  - Ravi Kumar P
FAU - Masoodi, Shariq
AU  - Masoodi S
FAU - Mukherjee, Kanchan Kumar
AU  - Mukherjee KK
FAU - Varma, Alka
AU  - Varma A
FAU - Kochhar, R
AU  - Kochhar R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pituitary
JT  - Pituitary
JID - 9814578
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Acromegaly/*complications/metabolism/*pathology
MH  - Adult
MH  - Apoptosis/physiology
MH  - Cell Proliferation
MH  - Colonic Neoplasms/metabolism/*pathology/*secondary
MH  - Female
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Middle Aged
EDAT- 2011/04/01 06:00
MHDA- 2012/09/22 06:00
CRDT- 2011/04/01 06:00
PHST- 2011/04/01 06:00 [entrez]
PHST- 2011/04/01 06:00 [pubmed]
PHST- 2012/09/22 06:00 [medline]
AID - 10.1007/s11102-011-0300-9 [doi]
PST - ppublish
SO  - Pituitary. 2012 Jun;15(2):166-73. doi: 10.1007/s11102-011-0300-9.