PMID- 21452228
OWN - NLM
STAT- MEDLINE
DCOM- 20120326
LR  - 20110426
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Linking)
VI  - 519
IP  - 9
DP  - 2011 Jun 15
TI  - Patterned expression of a cocaine- and amphetamine-regulated transcript peptide 
      reveals complex circuit topography in the rodent cerebellar cortex.
PG  - 1781-96
LID - 10.1002/cne.22601 [doi]
AB  - The cerebellum (Cb) of mammals and birds consists of an evolutionarily conserved 
      map defined by Purkinje cell (PC) protein expression. In mice, ZebrinII/aldolaseC 
      is expressed in a striking array of stripes in lobules I-V (anterior zone; AZ) 
      and VIII-anterior IX (posterior zone; PZ), whereas the small heat shock protein 
      25 (HSP25) is expressed in stripes in lobules VI-VII (central zone, CZ) and 
      posterior IX-X (nodular zone, NZ). Little is known about whether molecularly 
      defined afferent subsets terminate within specific PC stripes or whether their 
      topography is conserved across species. Using immunohistochemistry, we 
      demonstrate in adult mice and rats that cocaine- and amphetamine-regulated 
      transcript (CART) expression can be used to partition sensory-motor projections 
      into complex topographic maps. We found that in mice CART was expressed in 
      climbing fiber bands that generally corresponded to the pattern of 
      HSP25-expressing PCs in the CZ/NZ. In contrast, CART was expressed in climbing 
      fiber bands in all four transverse zones of the rat Cb. Within the rat AZ/PZ, 
      climbing fibers terminated selectively within the dendrites of 
      ZebrinII-immunoreactive PCs. In additional experiments, we observed CART 
      expression in loose clusters of spinocerebellar mossy fibers in the mouse AZ/PZ, 
      whereas in rat CART immunoreactive mossy fibers terminated predominantly in the 
      CZ/NZ. We conclude that, although the overall topography of CART-expressing 
      afferents is restricted within a conserved map of PC stripes and transverse 
      zones, their termination patterns also reflect species-specific compartmental 
      features.
CI  - Copyright (c) 2011 Wiley-Liss, Inc.
FAU - Reeber, Stacey L
AU  - Reeber SL
AD  - Dominick P. Purpura Department of Neuroscience, Albert Einstein College of 
      Medicine, Yeshiva University, Bronx, New York 10461, USA.
FAU - Sillitoe, Roy V
AU  - Sillitoe RV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
SB  - IM
MH  - Afferent Pathways/chemistry/cytology/metabolism
MH  - Animals
MH  - Axons/chemistry/metabolism
MH  - Body Patterning/physiology
MH  - Brain Mapping/methods
MH  - Cell Compartmentation/physiology
MH  - Cerebellar Cortex/*chemistry/cytology/*metabolism
MH  - Female
MH  - Male
MH  - Mice
MH  - Nerve Fibers/chemistry/metabolism
MH  - Nerve Tissue Proteins/*biosynthesis/chemistry/metabolism
MH  - Rats
MH  - Species Specificity
EDAT- 2011/04/01 06:00
MHDA- 2012/03/27 06:00
CRDT- 2011/04/01 06:00
PHST- 2011/04/01 06:00 [entrez]
PHST- 2011/04/01 06:00 [pubmed]
PHST- 2012/03/27 06:00 [medline]
AID - 10.1002/cne.22601 [doi]
PST - ppublish
SO  - J Comp Neurol. 2011 Jun 15;519(9):1781-96. doi: 10.1002/cne.22601.