PMID- 21453202
OWN - NLM
STAT- MEDLINE
DCOM- 20120302
LR  - 20220129
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Linking)
VI  - 27
IP  - 11
DP  - 2011 Nov
TI  - Short communication an interferon-gamma ELISPOT assay with two cytotoxic T cell 
      epitopes derived from HTLV-1 tax region 161-233 discriminates HTLV-1-associated 
      myelopathy/tropical spastic paraparesis patients from asymptomatic HTLV-1 
      carriers in a Peruvian population.
PG  - 1207-12
LID - 10.1089/AID.2011.0029 [doi]
AB  - HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic 
      and progressive disorder caused by the human T-lymphotropic virus type 1 
      (HTLV-1). In HTLV-1 infection, a strong cytotoxic T cell (CTL) response is 
      mounted against the immunodominant protein Tax. Previous studies carried out by 
      our group reported that increased IFN-gamma enzyme-linked immunospot (ELISPOT) 
      responses against the region spanning amino acids 161 to 233 of the Tax protein 
      were associated with HAM/TSP and increased HTLV-1 proviral load (PVL). An 
      exploratory study was conducted on 16 subjects with HAM/TSP, 13 asymptomatic 
      carriers (AC), and 10 HTLV-1-seronegative controls (SC) to map the 
      HAM/TSP-associated CTL epitopes within Tax region 161-233. The PVL of the 
      infected subjects was determined and the specific CTL response was evaluated with 
      a 6-h incubation IFN-gamma ELISPOT assay using peripheral blood mononuclear cells 
      (PBMCs) stimulated with 16 individual overlapping peptides covering the Tax 
      region 161-233. Other proinflammatory and Th1/Th2 cytokines were also quantified 
      in the supernatants by a flow cytometry multiplex assay. In addition, a set of 
      human leukocyte antigen (HLA) class I alleles that bind with high affinity to the 
      CTL epitopes of interest was determined using computational tools. Univariate 
      analyses identified an association between ELISPOT responses to two new CTL 
      epitopes, Tax 173-185 and Tax 181-193, and the presence of HAM/TSP as well as an 
      increased PVL. The HLA-A*6801 allele, which is predicted to bind to the Tax 
      181-193 peptide, was overpresented in the HAM/TSP patients tested.
FAU - Best, Ivan
AU  - Best I
AD  - Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana 
      Cayetano Heredia, Lima, Peru. ivan.best@upch.pe
FAU - Lopez, Giovanni
AU  - Lopez G
FAU - Talledo, Michael
AU  - Talledo M
FAU - MacNamara, Aidan
AU  - MacNamara A
FAU - Verdonck, Kristien
AU  - Verdonck K
FAU - Gonzalez, Elsa
AU  - Gonzalez E
FAU - Tipismana, Martin
AU  - Tipismana M
FAU - Asquith, Becca
AU  - Asquith B
FAU - Gotuzzo, Eduardo
AU  - Gotuzzo E
FAU - Vanham, Guido
AU  - Vanham G
FAU - Clark, Daniel
AU  - Clark D
LA  - eng
GR  - G0601072/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110509
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Gene Products, tax)
RN  - 0 (Peptides)
RN  - 0 (tax protein, Human T-lymphotrophic virus 1)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Carrier State/diagnosis/immunology/virology
MH  - Epitope Mapping
MH  - Epitopes, T-Lymphocyte/chemistry/*immunology
MH  - Female
MH  - Gene Products, tax/*chemistry/immunology
MH  - HTLV-I Infections/diagnosis/immunology/virology
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Interferon-gamma/*analysis
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Paraparesis, Tropical Spastic/*diagnosis/immunology/virology
MH  - Peptides/chemistry/*immunology
MH  - Peru
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Young Adult
EDAT- 2011/04/02 06:00
MHDA- 2012/03/03 06:00
CRDT- 2011/04/02 06:00
PHST- 2011/04/02 06:00 [entrez]
PHST- 2011/04/02 06:00 [pubmed]
PHST- 2012/03/03 06:00 [medline]
AID - 10.1089/AID.2011.0029 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2011 Nov;27(11):1207-12. doi: 10.1089/AID.2011.0029. 
      Epub 2011 May 9.