PMID- 21453728
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20181201
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 138
IP  - 1
DP  - 2011 Jul
TI  - A mixture of bacterial mechanical lysates is more efficient than single strain 
      lysate and of bacterial-derived soluble products for the induction of an 
      activating phenotype in human dendritic cells.
PG  - 86-91
LID - 10.1016/j.imlet.2011.03.006 [doi]
AB  - Dendritic cells (DCs), following an optimal maturation, are able to drive an 
      efficient immune-response. For this, both co-stimulatory molecules (CD80 and 
      CD86), activation molecules (CD83) and peptide presenting molecules (HLA) are 
      over-expressed. The in vitro treatment of immature DC with fragments of bacterial 
      strains, obtained by using a mechanical lysis as well as with bacterial-derived 
      molecules (such as lipopolysaccharide and protido-glycan), induced the maturation 
      of DCs and the secretion of a panel of cytokines and chemokines. Of note, ex vivo 
      treated circulating DCs and plasmacytoid DCs were also activated by these 
      bacterial bodies. However, while the particulate fraction of single bacterial 
      strains or soluble bacterial-derived molecules induced a sub-optimal maturation 
      (as evaluated by the expression of an activating phenotype on DCs and the amount 
      of cytokine secretion), the addition of the mixture of the particulate fractions 
      of the different bacterial strains was able to mediate an optimal maturation. 
      These results were also confirmed by using the secretion of both cytokines and 
      chemokines as markers of DC activation. All these findings suggest that the 
      particulate fraction of bacterial lysate mixtures, because of their ability to 
      interact with different surface structures, might be exploited not only as an 
      immunogen, but also as an adjuvant treatment to boost an immune-response to 
      poorly "antigenic" proteins, such as cancer antigens or allergens.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Morandi, Barbara
AU  - Morandi B
AD  - Laboratorio di Immunologia, Istituto Nazionale per la Ricerca sul Cancro, Genova, 
      Italy.
FAU - Agazzi, Alessia
AU  - Agazzi A
FAU - D'Agostino, Antonella
AU  - D'Agostino A
FAU - Antonini, Francesca
AU  - Antonini F
FAU - Costa, Gregorio
AU  - Costa G
FAU - Sabatini, Federica
AU  - Sabatini F
FAU - Ferlazzo, Guido
AU  - Ferlazzo G
FAU - Melioli, Giovanni
AU  - Melioli G
LA  - eng
PT  - Journal Article
DEP - 20110329
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antigens, Surface)
RN  - 0 (Broncho-Vaxom)
RN  - 0 (Cell Extracts)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigens, Surface/metabolism
MH  - Bacteria/*immunology
MH  - Cell Differentiation/immunology
MH  - Cell Extracts/*immunology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - *Phenotype
EDAT- 2011/04/02 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/04/02 06:00
PHST- 2010/09/07 00:00 [received]
PHST- 2011/02/15 00:00 [revised]
PHST- 2011/03/18 00:00 [accepted]
PHST- 2011/04/02 06:00 [entrez]
PHST- 2011/04/02 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - S0165-2478(11)00093-9 [pii]
AID - 10.1016/j.imlet.2011.03.006 [doi]
PST - ppublish
SO  - Immunol Lett. 2011 Jul;138(1):86-91. doi: 10.1016/j.imlet.2011.03.006. Epub 2011 
      Mar 29.