PMID- 21454863
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20110718
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 89
IP  - 8
DP  - 2011 Aug
TI  - Mannan oligosaccharide improves immune responses and growth efficiency of nursery 
      pigs experimentally infected with porcine reproductive and respiratory syndrome 
      virus.
PG  - 2592-602
LID - 10.2527/jas.2010-3208 [doi]
AB  - This study was conducted to determine whether the ingestion of mannan 
      oligosaccharide (MOS, Bio-Mos) alters the immune response of nursery pigs 
      challenged with porcine reproductive and respiratory syndrome virus (PRRSV). A 
      total of 64 pigs (3 wk old), free of PRRSV, were used in 2 separate but similar 
      experiments conducted sequentially. Pigs were blocked by initial BW. Sex and 
      ancestry were equalized across treatments. Pigs were randomly assigned from 
      within blocks to 1 of 4 treatments in a 2 x 2 factorial arrangement [2 types of 
      diet: control (0%) and MOS addition (0.2%); 2 levels of PRRSV: with and without]. 
      There were 8 replicate chambers of 2 pigs each. After 2 wk of a 4-wk period of 
      feeding the treatments, pigs were intranasally inoculated with PRRSV or a sterile 
      medium at 5 wk of age. The PRRSV challenge decreased ADG, ADFI, and G:F 
      throughout the experiment (P < 0.001). Feeding MOS improved G:F of the pigs 
      during d 7 to 14 (P=0.041) postinfection (PI). Serum concentrations of tumor 
      necrosis factor (TNF)-alpha, C-reactive protein, and haptoglobin were increased by 
      PRRSV (P < 0.001). The MOS x PRRSV interaction was significant for TNF-alpha at d 14 
      PI (P=0.028), suggesting that infected pigs fed MOS had less TNF-alpha than those fed 
      the control. Dietary MOS increased serum IL-10 at d 14 PI (P=0.036). Further, 
      MOS-fed pigs had greater numbers of white blood cells (WBC) at d 3 (P=0.048) and 
      7 PI (P=0.042) and lymphocytes at d 7 PI (P=0.023) than control-fed pigs. In 
      contrast, PRRSV decreased (P < 0.01) WBC numbers until d 14 PI. Dietary MOS 
      appeared (P=0.060) to increase the neutrophils in PRRSV-infected pigs at d 3 PI, 
      but no (P=0.202) MOS x PRRSV interaction was found. Infection with PRRSV 
      increased rectal temperature (RT) of pigs at d 3 PI (P < 0.001) and continued to 
      affect the infected pigs fed the control diet until d 14 PI. The MOS x PRRSV 
      interaction for RT was found at d 7 (P < 0.01) and 10 (P=0.098) PI, indicating 
      that the infected pigs fed MOS had a decreased RT compared with those fed the 
      control. This could explain why feed efficiency was improved by MOS. No effect (P 
      > 0.05) of treatments on viremia or PRRSV-specific antibody was observed. These 
      results suggest that MOS is associated with rapidly increased numbers of WBC at 
      the early stage of infection and alleviates PRRSV-induced effects on G:F and 
      fever. The results also indicate that the reduced intensity of inflammation by 
      MOS may be related to changes in inflammatory mediator levels at the end of the 
      acute phase.
CI  - (c) 2011 American Society of Animal Science. All rights reserved.
FAU - Che, T M
AU  - Che TM
AD  - Department of Animal Sciences, University of Illinois, Urbana 61801, USA. 
      chetung2@illinois.edu
FAU - Johnson, R W
AU  - Johnson RW
FAU - Kelley, K W
AU  - Kelley KW
FAU - Van Alstine, W G
AU  - Van Alstine WG
FAU - Dawson, K A
AU  - Dawson KA
FAU - Moran, C A
AU  - Moran CA
FAU - Pettigrew, J E
AU  - Pettigrew JE
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20110331
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Mannans)
SB  - IM
MH  - Acute-Phase Proteins/metabolism
MH  - Animal Feed/analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Cytokines/blood/metabolism
MH  - Diet/veterinary
MH  - Female
MH  - Leukocyte Count
MH  - Male
MH  - Mannans/chemistry/*pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*immunology
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - Time Factors
EDAT- 2011/04/02 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/04/02 06:00
PHST- 2011/04/02 06:00 [entrez]
PHST- 2011/04/02 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
AID - jas.2010-3208 [pii]
AID - 10.2527/jas.2010-3208 [doi]
PST - ppublish
SO  - J Anim Sci. 2011 Aug;89(8):2592-602. doi: 10.2527/jas.2010-3208. Epub 2011 Mar 
      31.