PMID- 21455985
OWN - NLM
STAT- MEDLINE
DCOM- 20120227
LR  - 20160303
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 130
IP  - 4
DP  - 2012 Feb 15
TI  - The gene expression profile of unstimulated dendritic cells can be used as a 
      predictor of function.
PG  - 979-90
LID - 10.1002/ijc.26101 [doi]
AB  - Dendritic cells (DCs) represent a subset of professional antigen presenting cell 
      (APC) whose role is to elicit immune responses against harmful antigens. They 
      have been used in DC vaccines to stimulate the immune system to kill cancer 
      cells. However, successes in clinical trials have been limited, which may be 
      attributed to a lack of appreciation of the quality of DCs used. In the present 
      study, whole human genome microarrays were used to examine alterations in gene 
      expression of monocyte-derived DCs after stimulation with supernatants derived 
      from tumours. Our primary aim was to investigate the possibility of a gene 
      signature for DCs that could be used to forecast responsiveness to tumour 
      stimuli. Results showed that DCs are divided into two groups based on their 
      ability to increase costimulatory markers and to trigger T-cell responses. The 
      gene profiles of the immature DCs from these two groups were distinct, with 
      particular divergence in genes from the interleukin (IL) 8 and thrombospondin-1 
      hubs. A subpanel of genes was identified, whose signature of expression was 
      capable of predicting DC-stimulatory capacity. Overall, these studies have 
      highlighted a gene-based screen that predicts DC function, which could be used to 
      guide DC-vaccine trials.
CI  - Copyright (c) 2011 UICC.
FAU - Liu, Wai M
AU  - Liu WM
AD  - Department of Oncology, Division of Clinical Sciences, St George's University of 
      London, London, United Kingdom. w.liu@sgul.ac.uk
FAU - Dennis, Jayne L
AU  - Dennis JL
FAU - Fowler, Daniel W
AU  - Fowler DW
FAU - Dalgleish, Angus G
AU  - Dalgleish AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110621
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Vaccines)
SB  - IM
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology/metabolism
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Neoplasms/immunology/therapy
MH  - Oligonucleotide Array Sequence Analysis
MH  - Vaccines/immunology
EDAT- 2011/04/02 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/04/02 06:00
PHST- 2011/02/22 00:00 [received]
PHST- 2011/03/23 00:00 [accepted]
PHST- 2011/04/02 06:00 [entrez]
PHST- 2011/04/02 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - 10.1002/ijc.26101 [doi]
PST - ppublish
SO  - Int J Cancer. 2012 Feb 15;130(4):979-90. doi: 10.1002/ijc.26101. Epub 2011 Jun 
      21.