PMID- 214582
OWN - NLM
STAT- MEDLINE
DCOM- 19790221
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 28
IP  - 2
DP  - 1978 Nov
TI  - Modulation of mouse mammary tumor virus production in the MJY-alpha cell line.
PG  - 611-23
AB  - Implantation of the mouse mammary tumor virus (MMTV)-producing mammary tumor cell 
      line MJY-alpha into isogeneic mice elicited both humoral and T-cell responses 
      against MMTV virion antigens. The carcinosarcomas which developed from the 
      implanted cells showed a significant decrease in MMTV synthesis, compared with 
      cells remaining in culture, which was detectable as early as 7 days after 
      implantation and for five transplant generations. Electron microscopic 
      examination of thin sections of the tumors revealed that intracytoplasmic A 
      particles, budding particles, and cell-free MMTV B particles were all affected. 
      However, immunofluorescence assays of tumor sections demonstrated the presence of 
      MMTV viral antigens in the cells. Cell cultures initiated from first-, third-, 
      and fourth-generation tumors were morphologically identical to the original in 
      vitro cell line, although virus production was barely detectable. Analysis of the 
      cultures by electron microscopy revealed a significant increase in MMTV virions 
      after in vitro passage 3. Polypeptide profiles obtained by sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis of virions purified from these 
      cultures were identical to MMTV. Immunodiffusion demonstrated the 
      cross-reactivity between these virions and MMTV particles obtained from mouse 
      milk. In vitro treatment of MJY-alpha cell cultures with rabbit anti-MMTV 
      antiserum resulted in a reduction of extracellular MMTV virions, as well as 
      alterations in their sodium dodecyl sulfate-polyacrylamide gel electrophoretic 
      polypeptide patterns.
FAU - Yagi, M J
AU  - Yagi MJ
FAU - Blair, P B
AU  - Blair PB
FAU - Lane, M A
AU  - Lane MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Cell Line
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Immune Sera/pharmacology
MH  - Mammary Neoplasms, Experimental/*immunology
MH  - Mammary Tumor Virus, Mouse/*growth & development/immunology
MH  - Mice
MH  - Neoplasm Transplantation
MH  - *Virus Replication
PMC - PMC354309
EDAT- 1978/11/01 00:00
MHDA- 1978/11/01 00:01
PMCR- 1978/11/01
CRDT- 1978/11/01 00:00
PHST- 1978/11/01 00:00 [pubmed]
PHST- 1978/11/01 00:01 [medline]
PHST- 1978/11/01 00:00 [entrez]
PHST- 1978/11/01 00:00 [pmc-release]
AID - 10.1128/JVI.28.2.611-623.1978 [doi]
PST - ppublish
SO  - J Virol. 1978 Nov;28(2):611-23. doi: 10.1128/JVI.28.2.611-623.1978.